Protection of phosphatidylcholine to photosystemⅡmembrane during heat treatment  

Protection of phosphatidyl-choline to photosystem II membrane during heat treatment

作　　者：LIDonghai XUQiang GONGYandao ZHANGXiufang ZHAONanming WANGKebin KUANGTingyun 

机构地区：[1]StateKeyLaboratoryofBiomembraneandMembraneBiotechnology,DepartmentofBiologicalSciencesandBiotecbnology,TsingbuaUniversity,Beijing100084,China [2]PhotosynthesisResearchCenter,InstituteofBotany,ChineseAcademyofSciences,Beijing100093,China

出　　处：《Chinese Science Bulletin》2003年第9期897-901,共5页

基　　金：supported by the State Key Basic Research and Development Program(Grant No.G1998010100);the National Natural Science Foundation of China(Grant No.39890390).

摘　　要：Photosystem Ⅱ membrane was reconstituted with phosphatidylcholine (PC) with different kinds of fatty acyl chains and the protection of PC to photosystem Ⅱ (PSⅡ) membrane during heat treatment was investigated using oxygen electrode, variable fluorescence and circular dichroism (CD) spectroscopy. Heat treatment decreased the oxygen evolution rate and the F′v/Fm′ ratio of PSⅡ membrane and influenced CD spectra of PSⅡ membrane, but PC inhibited the effect of heat treatment on the oxygen evolution rate, the F′v/F′m ratio and CD spectra of PSⅡ membrane. The results indicate that PC can protect PSⅡ membrane against heat treatment and the alterations in the unsaturated fatty acid extent in PC can cause the changes of the protection ability.Photosystem II membrane was reconstituted with phosphatidylcholine (PC) with different kinds of fatty acyl chains and the protection of PC to photosystem II (PS II) membrane during heat treatment was investigated using oxygen electrode, variable fluorescence and circular dichro-ism (CD) spectroscopy. Heat treatment decreased the oxygen evolution rate and the F'v/Fm' ratio of PS II membrane and influenced CD spectra of PS II membrane, but PC inhibited the effect of heat treatment on the oxygen evolution rate, the F'v/F'm ratio and CD spectra of PS II membrane. The results indicate that PC can protect PS II membrane against heat treatment and the alterations in the unsaturated fatty acid extent in PC can cause the changes of the protection ability.

关 键 词：卵磷脂保护 光合体系Ⅱ薄膜 循环二色性 氧进化速率 热疗法 可变荧光性 

分 类 号：Q947.5[生物学—植物学] Q945.11