Characterization and mapping of a lesion mimic mutant in rice (Oryza sativa L.)  

作　　者：LIUDaofeng CHENGZhukuan LIUGuoqing LIUGuozhen WANGYun ZHAOXianfeng ZHULihuang 

机构地区：[1]InstituteofGeneticsandDevelopmentalBiology,ChineseAcademyofSciences,Beijing100101,China [2]HebeiAgricultureUniversity,Baoding071000,China

出　　处：《Chinese Science Bulletin》2003年第9期892-896,共5页

基　　金：This work was supported by the Knowledge Innovation Program of the Chinese Academy of Sciences(Grant No.KXCXZ-1-02-01);the National Natural Science Foundation of China(Grant No.39880020).

摘　　要：A rice initiation-type lesion mimic mutant (imi) was identified, which was isolated from an indica rice Zhongxian 3037 through γ radiation mutagenesis. Trypan blue staining and sterile culture revealed that the mutant spontaneously developed lesions on the leaves in a develop mentally regulated and light-dependent manner. Genetic analysis indicated that the lesion mimic trait was controlled by a single resessive locus. Using public molecular markers and an F2 population derived from lmi and 93-11, we mapped the lmi locus to the short arm of chromosome 8, nearby the centromere, between two SSR markers RM547 and RM331. The genetic distance was 1.2 and 3.2 Cm, respectively. Then according to the public rice genomic sequence between the two SSR markers, lmi was further finely tagged by three CAPS markers: C4135-8, C4135-9 and C4135-10. And lmi locus was a co-segregated with marker C4135-10, providing a starting point for imi gene cloning.A rice initiation-type lesion mimic mutant (lmi) was identified, which was isolated from an indica rice Zhongxian 3037 through y radiation mutagenesis. Trypan blue staining and sterile culture revealed that the mutant spontaneously developed lesions on the leaves in a develop-mentally regulated and light-dependent manner. Genetic analysis indicated that the lesion mimic trait was controlled by a single resessive locus. Using public molecular markers and an F2 population derived from lmi and 93-11, we mapped the lmi locus to the short arm of chromosome 8, nearby the centromere, between two SSR markers RM547 and RM331. The genetic distance was 1.2 and 3.2 cM, respectively. Then according to the public rice genomic sequence between the two SSR markers, lmi was further finely tagged by three CAPS markers: C4135-8, C4135-9 and C4135-10. And lmi locus was a co-segregated with marker C4135-10, providing a starting point for lmi gene cloning.

关 键 词：ORYZA SATIVA L. 水稻 损害模仿突变基因 基因绘制 辐射变异 

分 类 号：S511[农业科学—作物学] Q78[生物学—分子生物学]