海马脑片LTP中胞内钙变化及硫氮卓酮的影响  

EFFECT OF DILTIAZEM ON CHANGES OF INTRACELLULAR CALCIUM IN AREA CA_1 OF THE HIPPOCAMPAL SLICE

作　　者：胡志安[1] 陈火明[2] 罗峻[1] 黎海蒂[1] 刘祚周[1] 余争平[3] 

机构地区：[1]第三军医大学生理学教研室 [2]第三军医大学第二炮兵总医院神经外科,北京100088 [3]第三军医大学劳动卫生学教研室,重庆400038

出　　处：《神经解剖学杂志》2004年第2期143-146,共4页Chinese Journal of Neuroanatomy

基　　金：国家自然科学基金 (No. 3 960 0 0 48;3 0 2 70 44 3 )资助项目

摘　　要：本文利用脑片胞内荧光钙指示剂 F ura-3标记和场电位测定技术 ,观察了海马 CA1 区 NMDA受体依赖型长时程增强(L TP)中钙离子变化及 L型钙通道阻断剂硫氮卓酮的影响。结果发现 ,条件刺激在诱出 L TP(NMDA受体依赖型 )产生的同时 ,胞内钙荧光强度显著升高。激光共聚焦显微镜下 ,可见 CA1 区锥体细胞轮廓明显。而海马其它各区胞内钙荧光强度无显著变化。在 LTP维持期 ,胞内钙荧光强度出现显著下降。硫氮卓酮 (3 0μmol/ L)显著抑制 CA1 区 L TP诱出和胞内钙荧光强度升高。这些结果表明 ,在海马 CA1 区 NMDA受体依赖型 LTP中 ,LCombining the use of the fluorescent calcium indicator Fura 3 with field potential measurements, we have observed the effect of the L type calcium channel blocker diltiazem on the intracelluar calcium transients in long term potentiation (LTP) in area CA 1 of the hippocampal slice preparation. Conditioning stimulation resulted in NMDA receptor dependent LTP and elicited a cytoplasmic free Ca 2+ concentration rise in pyramidal cell dendrites in area CA 1. Soma and dendrites of pyramidal cell could be clearly distinguished using confocal laser scanning microscope. Whereas there were no changes of density of Ca 2+ fluorescence in the other regions of hippocampus. In addition, density of Ca 2+ fluorescence decresed markedly in CA 1 area during LTP maintenance. LTP was prevented and calcium transients were depressed in the presence of the L type calcium channel blocker diltiazem. These results indicate that L type calcium channels may play a key role in NMDA receptor dependent LTP in area CA 1 of the hippocampus.

关 键 词：海马脑片 LTP 胞内钙 硫氮卓酮 激光共聚焦显微镜 钙通道 

分 类 号：R33[医药卫生—人体生理学]