LDFF,the large molecular weight DNA fragmentation factor,is responsible for the large molecular weight DNA degradation during apoptosis in Xenopus egg extracts  

作　　者：ZhiGangLU ChuanMaoZHANG ZhongHeZHAI 

机构地区：[1]DepartmentofCellBiologyandGenetics,CollegeofLifeSciences,PekingUniversity,Beijing100871,China.

出　　处：《Cell Research》2004年第2期134-140,共7页细胞研究（英文版）

基　　金：This work was supported by Major State Basic Research Program of China(No.G1999053905);National Science Fund for Distinguished Young Scholars(No.30225016).

摘　　要：DNA degradation is a biochemical hallmark in apoptosis. It has been demonstrated in many cell types that there are two stages of DNA fragmentation during the apoptotic execution. In the early stage, chromatin DNA is cut into large molecular weight DNA fragments, although the responsible nuclease(s) has not been recognized. In the late stage, the chromatin DNA is cleaved further into short oligonucleosomal fragments by a well-characterized nuclease in apoptosis,the caspase-activated DNase (CAD/DFF40). In this study, we demonstrate that large molecular weight DNA fragmentation also occurs in Xenopus egg extracts in apoptosis. We show that the large molecular weight DNA fragmentation factor (LDFF) is not the Xenopus CAD homolog XCAD. LDFF is activated by caspase-3. The large molecular weight DNA fragmentation activity of LDFF is Mg2+-dependent and Ca2+-independent, can occur in both acidic and neutral pH conditions and can tolerate 45℃ treatment. These results indicate that LDFF in Xenopus egg extracts might be a new DNase (or DNases) responsible for the large DNA fragmentation.

关 键 词：APOPTOSIS caspase-activated DNase (CAD) large molecular weight DNA fragmentation factor (LDFF). 

分 类 号：Q523[生物学—生物化学] Q255