一体化微流控芯片的酶固定化技术  

Enzyme Immobilization Technique in Microfluidic Channel on Integrated Chip

作　　者：田玉平[1] 吴会灵[2] 陈淑桂[1] 杨芃原[2] 

机构地区：[1]吉林大学化学学院,长春130026 [2]复旦大学化学系,上海200433

出　　处：《高等学校化学学报》2004年第5期847-849,共3页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金 (批准号 :2 0 2 990 3 0 );国家"九七三"计划项目 (批准号:0 0 1CB5 10 2 0 2 );国家"八六三"计划项目 (批准号 :2002BAC11A11)资助

摘　　要：A new method for the immobilization of enzyme in microfluidic channel on the integrated chip for proteomic analysis was reported. The PDMS film immersing in the acrylic acid solution was irradiated under UV lamp to produce active free radicals and then immersed alternatively into the PDDA solution and trypsin solution for self-assemble. The monomer solution of the produced chip was characterized with the reflectance IR technique, contact angle determination and SEM observations. A standard protein BSA was selected to test the performance of the product. It was verified that CE and MALDI-TOF/TOF-MS detection can be on-line carried out in a single, successive running.A new method for the immobilization of enzyme in microfluidic channel on the integrated chip for proteomic analysis was reported. The PDMS film immersing in the acrylic acid solution was irradiated under UV lamp to produce active free radicals and then immersed alternatively into the PDDA solution and trypsin solution for self-assemble. The monomer solution of the produced chip was characterized with the reflectance IR technique, contact angle determination and SEM observations. A standard protein BSA was selected to test the performance of the product. It was verified that CE and MALDI-TOF/TOF-MS detection can be on-line carried out in a single, successive running.

关 键 词：聚二甲基硅氧烷 一体化微流控芯片 酶固定化技术 微全分析系统 

分 类 号：O657[理学—分析化学]