1株具抑制一氧化氮合成酶活性放线菌菌株的分离和鉴定  

Isolation and identification of one actinomycete strain with strong activity for restraining NO synthesize enzyme

作　　者：李文均[1] 姜怡[1] 王栋[1] Reiner M Kroppenstedt 徐丽华[1] 姜成林[1] 

机构地区：[1]云南大学云南省微生物研究所教育部微生物资源开放研究重点实验室,云南昆明650091 [2]Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH

出　　处：《云南大学学报（自然科学版）》2004年第3期256-260,共5页Journal of Yunnan University(Natural Sciences Edition)

基　　金：国家科技部基础研究重大项目(2001CCC00600);国家自然科学基金资助项目(30270004);云南省自然科学基金资助项目(2001C0001Q);云南省教育厅基金资助项目(01111134;02QJ077).

摘　　要：从云南河口采集的土壤中分离到1株稀有放线菌YIM30243T.该菌株具有很强的抑制一氧化氮(NO)合成酶活性.通过对其进行的包括形态、生理生化特性、细胞壁化学组分以及16SrDNA序列等方面的多相分类研究,确定其为诺卡氏菌属的1个新种,我们将其命名为白色诺卡氏菌(Nocardiaalbasp.nov.).该菌株在大多数培养基上生长良好,气生菌丝呈黄白色至粉红白色,基内菌丝黄白至黄褐色.气生菌丝、基内菌丝均呈不规则断裂.One rare actinomycete strain,which had strong activity for restraining NO synthesize enzyme,isolated from a soil sample collected from Hekou,Yunnan Province.Based on our polyphasic studies,including its morphology,physiological and biochemical characteristics,chemotaxonomy and 16S rDNA sequence analysis,it was proposed it as a new species of genus Nocardia and named it as Nocardia alba sp.nov..Strain YIM 30243~T grew well on most test media.The color of the aerial mycelium were from yellow-white to pink-white,the substrate mycelium were from yellow-white to yellow-brown, and the aerial and substrate mycelium all irregularly fragmented.

关 键 词：一氧化氮合成酶 诺卡氏菌 稀有放线菌 16S RDNA NOS 药物 

分 类 号：R284[医药卫生—中药学] Q939.132[医药卫生—中医学]