人生长激素LAB-ELISA测定方法  

MEASUREMENT OF HUMAN GROWTH HORMONE: DEVELOPMENT AND VALIDATION OF A SENSITIVE AND SPECIFIC ASSAY

作　　者：黄为群 汪恩壁[1] 郭礼和[1] 程有红 王德芬[2] 曾畿生[2] 

机构地区：[1]中国科学院上海细胞生物学研究所,200031 [2]上海第二医科大学附属瑞金医院儿科

出　　处：《中华内分泌代谢杂志》1992年第1期38-40,共3页Chinese Journal of Endocrinology and Metabolism

摘　　要：本文报道了用LAB-ELISA技术测定尿中人生长激素的方法。本方法对人催乳素、人促甲状腺素、人促卵泡成熟激素和人促黄体生成激素均无明显交叉反应。精密度测试批内CV 4.72～12.00％,批间CV 4.89～11.17％,偏倚为0.20～12.00％,回收率达100±1.4％。本文对LAB-ELISA技术所涉及的因素作了较系统的研究,并提出了优化条件。通过LAB-ELISA技术测定正常和GHD儿童的尿标本,表明本测定技术具有灵敏度高、特异性强和稳定性好等优点。The determination of human growth hormor.e (hGH) in urine by means of LAB-ELISA technique was described. The major factors affecting the Sensitivity of LAB-ELISA were studied systematically. The optimized conditions have been established. The sensitivity of LAB-ELISA is at the level of pg/ml. There were little cross binding with human prolactin, thyroid-stimulating hormone, follicular-stimulating hormone, and luteinizing hormone. Human growth hormone levels in overnight urine in 50 normal children and 11 cases of GH-deficiency (GHD) were measurejl by the LAB-ELISA technique. Results of recovery test (recovery rate 100 ±1.4%) and precision test (intra-assay CV 4.72-12.00%, inter-assay CV 4.89-11.17%, and bias 0.20-12.00%)indicate that the method is highly specific,precise and stable.

关 键 词：生物素 亲和素 生长激素 ELISA 

分 类 号：R446.61[医药卫生—诊断学]