合成的人血浆甲状旁腺素(1-34)放射免疫测定法及其临床应用  

RIA OF HUMAN PLASMA PARATHYROID HORMONE_(1-34) AND ITS CLINICAL APPLICATION

作　　者：徐志惠[1] 季冠芳[1] 苏学良[1] 方佩华[1] 谭郁彬[1] 

机构地区：[1]天津市内分泌研究所,300070

出　　处：《中华内分泌代谢杂志》1992年第3期133-134,共2页Chinese Journal of Endocrinology and Metabolism

摘　　要：用合成的人甲状旁腺素(1-34)肽段(hPTH_(1-34))免疫动物,产生特异抗体,制备标记物,建立了放射免疫测定法(RIA)。不同hPTH_(1-34)浓度平均回收率为93.0％,灵敏度为1.1pmol/L,批内和批间变异系数分别为6.0％及13.0％。与另外3种肽段的交叉反应均<0.05％。测定100例正常受试者,生理参考值为11.8±4.5pmol/L。与用hPTH_(1-84)和PTH_(44-68)肽段进行比较,该法具有很好相关性,可用于临床诊断。A RIA method for determining human plasma parathyroid hormone (hPTH1-34) was established. This was possible mainly by the production of an antiserum of high liter raised in rabbit against hPTH1-34. The methodological investigation data were listed below: the affinity constant, Ka= 1,95×1010 mol/L; the cross reactivity with each of the three analogous peptides, i.e., hACTH1-34, hCT and hPTH44_68, being all less than 0.005%; the intra- and inter-batch coefficients of variation being 6.0% and 13.0% respectively; the recoveries for different levels of hPTH1_34 being ranged within 83.1 - 102.6%; and the sensitivity being 1.1 pmol/L. The reference value obtained by testing 50 male and 50 female normal adults was 11.7 ±4.4 pmol/L. It has been applied clinically for patients with various endocrine diseases including hyper- and hypoparathyroidisms and metabolic bone diseases especially in the aged population and the results fitted well with clinical diagnosis.

关 键 词：放射免疫测定 甲状旁腺素 临床 

分 类 号：R977.14[医药卫生—药品]