脑钠素和脑钠素类似物的生物活性  

STUDY ON THE BIOLOGICAL ACTIVITIES OF BRAIN NATRIURETIC PEPTIDE AND ITS ANALOG

作　　者：盛树力[1] 张万江[1] 伍洁[2] 刘爱民 

机构地区：[1]北京宣武医院,100050 [2]北京市儿童医院 [3]北京市神经外科研究所

出　　处：《中华内分泌代谢杂志》1992年第3期161-163,共3页Chinese Journal of Endocrinology and Metabolism

摘　　要：应用自己合成的脑钠素(BNP)和脑钠素类似物(BNP-A),研究了它们的生物活性。研究结果如下:(1)在低渗葡萄糖溶液极度扩增大鼠血容量时,脑室内注入BNP仍有明显利钠作用,但对输入5％或10％葡萄糖溶液的大鼠无明显的利钠利尿的累加效应;(2)60分钟内静脉滴入BNP或BNP-A能明显降低自发性高血压大鼠血管紧张素I和醛固酮浓度,但一次大剂量给予BNP却使血管紧张素I浓度明显增加,醛固酮浓度正常。这可能是激素分泌抑制后的反跳现象;(3)体外实验表明,BNP扩张血管的效果不如降钙素基因相关肽(CGRP),但在体内增加局部脑血流的效果与CGRP相仿。实验结果表明,BNP是一种调节水盐代谢和局部脑血流的重要多肽。Brain natriuretic peptide consists of 26 amino acids and has diuretic, natriuretic and hypotensive activities. We have synthesized BNP and its analog (BNP-A) (Mpr4, D-Ala613)-BNP(4-26)-NH2 using solid phase method and examined their biological activities. Our results showed that 1) BNP (10-7mol/L) dilated human basal cerebral artery which was pre-constricted with endothelin, 5-HT and noradrenaline in vitro; 2) both BNP (5 μg/ rat) and BNP-A (5 μg/ rat) increased regional cerebral blood flow following i.v. injection in rats; 3) intracerebroventricular injection of BNP (3 μg/ rat) induced an obvious natriuretic effect when blood volume was extremely expanded with hypoosmotic glucose solution in rats; 4) BNP (5 μg/ rat) infusion for 60 minutes caused a decline of plasma concentration of angiotensin II and aldosterone at the end of infusion in spontaneous hypertensive rats (SHR), and the bolus injection of BNP (10 μg/ rat) led to a fall in blood pressure about 1.33kPa. We conclude that BNP is an important peptide in regulating water and sodium metabolism and plays an important role in regulating blood flow to various organs.

关 键 词：脑钠素 脑钠素类似物 生物活性 

分 类 号：R972.4[医药卫生—药品]