牙鲆血清中免疫球蛋白M的分离及其测定  

Isolation and detection of Japanese flounder mmunoglobulin M

作　　者：王先磊[1] 张培军[1] 李军[1] 徐永立[1] 

机构地区：[1]中国科学院海洋研究所实验海洋生物学开放研究实验室,山东青岛266071

出　　处：《海洋科学》2004年第4期8-12,共5页Marine Sciences

基　　金：国家973 课题(G1999012006)

摘　　要：通过提取健康养殖牙鲆(Paralichthys　olivaceus)血清中的免疫球蛋白(Ig),并测定其相对分子质量,其中重链(H链)的相对分子质量为74.2　ku,轻链(L链)的相对分子质量为23.8　ku。用纯化的牙鲆血清中的Ig作抗原,制备多克隆抗体,确立牙鲆血清中免疫球蛋白的最佳测定方法。The serum IgM of healthy cultured flounder was purified and the molecule of the IgM was measured. The height chain (H chain) molecule weight and the light chain (L chain) molecule weight of the purified serum IgM were 74.2 ku and 23.8 ku. The polyclonal antibodies anti-serum IgM of flounder can be obtained by injecting the animals with the purified IgM . This study provided a rellable method of measuring the concentration of the serum IgM in fish.

关 键 词：牙鲆 血清 免疫球蛋白M IGM ELISA 抗原 多克隆抗体 

分 类 号：Q511[生物学—生物化学] Q959.486