去细胞异体神经和碱性成纤维细胞生长因子修复神经缺损  

Acceleraton of nerve regeneration following acellular allogeneic nerve grafting by bFGF

作　　者：李跃军[1] 陈绍宗[1] 李学拥[1] 李望舟[1] 

机构地区：[1]第四军医大学唐都医院整形外科,西安市710038

出　　处：《中华显微外科杂志》2004年第1期38-39,F004,共3页Chinese Journal of Microsurgery

基　　金：唐都医院苗子人才基金资助 (2 0 0 1 0 5)

摘　　要：目的　研究碱性成纤维细胞生长因子 (bFGF)对去细胞异体神经修复神经缺损的促进作用。　方法　根据bFGF因子用药浓度分为 10 0 0、5 0 0、2 5 0和 10 0U/ml与盐水对照组每组 5只日本大耳白兔。采用抗神经微丝免疫组化染色观察各组神经纤维再生的距离 ,抗S 10 0免疫组化染色观察许旺细胞的分布。研究不同浓度和剂量的外源性bFGF对去细胞支架移植后早期神经再生的影响。　结果　高浓度bFGF组 (10 0 0U/ml和 5 0 0U/ml)的神经再生距离在术后 10d明显大于盐水对照组 ,(P <0 0 1)。而低浓度bFGF组 (2 5 0U/ml和 10 0U/ml)与盐水对照组无明显差别 (P >0 0 5 )。　结论　去细胞异体神经移植后早期应用一定浓度和剂量的bFGF能明显提高再生轴突在支架内的生长速度。Objective To evaluate the promoting effect by bFGF on nerve regeneration following acellular allogeneic nerve grafting,and the effects of various concentration of bFGF on axon growth. Methods The animals were divided into 1 000U/ml, 500U/ml, 250U/ml,100U/ml and normal saline groups. Immunohistochemistry staining of neurofilament-160 and S-100 was used to show the length of axonal growth and Schwann cell infiltration 10 days after the surgery. Results The average distance of regenerated nerve fibers in the high dose groups (1 000U/ml and 500U/ml) was longer than that in the normal saline control group;but there was no difference between the low dose groups (250U/ml and 100U/ml) and the control group. Conclusion Early use of bFGF when acellular allogeneic nerve grafted to bridge peripheral nerve defect may obviously promote axon growth speed.

关 键 词：去细胞异体神经 碱性成纤维细胞生长因子 神经修复 神经缺损 免疫组织化学 组织工程学 

分 类 号：R651.3[医药卫生—外科学]