辅助电极对纳升电喷雾影响的研究  

Influence of Auxiliary Electrode on Nanoliter Electrospray

作　　者：许雪姣[1] 翟建军[1] 水雯箐[1] 徐国宾[1] 杨芃原[1] 

机构地区：[1]复旦大学化学系,上海200433

出　　处：《高等学校化学学报》2004年第4期627-629,共3页Chemical Journal of Chinese Universities

基　　金：国家"九七三"项目 (批准号 :0 0 1CB5 10 2 0 2 );"八六三"项目 (批准号 :2 0 0 1AA2 3 3 0 3 1);国家自然科学基金 (批准号 :2 0 2 990 3 0 )资助

摘　　要：An auxiliary electrode was used to improve the electric field distribution between the nESI emitter and the MS sampler. The results show an improvement in the ratio of signal to noise for nanoliter electrospray using a home-made nanoliter electrospray tip, evaluated by spraying a 25 pg/μL reserpine solution. In addition, the average ratios of signal to noise of reserpine acquired within 30 min at different concentrations were compared with those obtained with an auxiliary electrode. The experimental results suggest that an auxiliary electrode could dramatically improve the ratio of signal to noise and provide confident trends for future application of auxiliary electrode in nanoliter electrospray.An auxiliary electrode was used to improve the electric field distribution between the nESI emitter and the MS sampler. The results show an improvement in the ratio of signal to noise for nanoliter electrospray using a home-made nanoliter electrospray tip, evaluated by spraying a 25 pg/μL reserpine solution. In addition, the average ratios of signal to noise of reserpine acquired within 30 min at different concentrations were compared with those obtained with an auxiliary electrode. The experimental results suggest that an auxiliary electrode could dramatically improve the ratio of signal to noise and provide confident trends for future application of auxiliary electrode in nanoliter electrospray.

关 键 词：电喷雾质谱 纳升电喷雾技术 辅助电极 信噪比 利血平 稳定性 重现性 蛋白质组学 

分 类 号：O657.63[理学—分析化学] Q51[理学—化学]