N-苯甲氧羰基-L-色氨酸为模板的单分散分子印迹聚合物微粒的制备  

Preparation of Uniform-sized Molecularly Imprinted PolymerMicrospheres Using N-Cbz-L-Trp as Template

作　　者：郭天瑛[1] 张丽影[1] 郝广杰[1] 宋谋道[1] 张邦华[1] 

机构地区：[1]南开大学高分子化学研究所,吸附分离功能高分子材料国家重点实验室,天津300071

出　　处：《高等学校化学学报》2004年第4期762-765,共4页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金 (批准号 :5 0 0 0 3 0 0 6)资助

摘　　要：采用两步溶胀与悬浮聚合联用方法 ,以 2 -乙烯基吡啶 ( 2 -VP)为功能单体 ,二乙二醇二丙烯酸酯( DEGDA)为交联剂 ,成功制备出以 N-苯甲氧羰基 -L-色氨酸 ( N-Cbz-L-Trp)为模板的单分散分子印迹聚合物 ,并用扫描电镜、氮气吸附、拉曼光谱、高效液相色谱、热失重分析等测试手段进行表征 .结果表明 ,分子印迹聚合物的平均粒径为 6.3 μm,多分散系数为 1 .0 3 .拉曼光谱显示聚合物反应完全 ,模板分子洗脱充分 .高效液相色谱表征显示 ,分子印迹聚合物在很短的色谱柱中即可实现对印迹分子对映异构体的基线分离 .Uniform-sized molecularly imprinted polymer microspheres were prepared by using N-benzyloxycarbonyl-L-tryptophane(N-Cbz-L-Trp) as the template via a multi-step swelling and suspension polymerization method in which 2-vinylpyridine(2-VP) was used as the functional monomer and diethylene glycol diacrylate(DEGDA) was used as the cross-linker. The surfaces of nonporous micropheres were rough. The average diameter of polymer micropheres was 6.3 μm, and its distribution coeffiene was 1.03. It was found that the baseline chiral separation of the template enantiomer was achieved on a shorter column. Moreover, the removing of the template molecule and the thermal stability of MIP micropheres were also discussed.

关 键 词：N-苯甲氧羰基-L-色氨酸 单分散分子印迹聚合物 微粒 制备 两步溶胀法 悬浮聚合法 氨基酸衍生物 2-乙烯基吡啶 色谱固定相 

分 类 号：O657.7[理学—分析化学] O633.2[理学—化学]