巢蛋白在大鼠胚胎、新生及成年胰腺中的表达变化  

Expression and location of nestin in pancreas of E18.5, newborn and adult rats

作　　者：倪雪峰[1] 袁栎[1] 程志祥[1] 熊俊[1] 顾冬民[1] 朱自路[1] 周锦勇[1] 仲燕[1] 德伟[1] 

机构地区：[1]南京医科大学生物化学和分子生物学系,江苏南京210029

出　　处：《第四军医大学学报》2004年第3期193-196,共4页Journal of the Fourth Military Medical University

基　　金：江苏省自然科学基金 (BK2 0 0 1 1 1 9)

摘　　要：目的 :探讨巢蛋白 (nestin)在胚胎后期、新生期、成年三阶段的核酸、蛋白水平表达的变化 .方法 :取三个发育时期的胰腺 ,应用RT PCR、免疫组化的方法进行半定量、定位分析 .结果 :在胚胎后期、新生期、成年三阶段nestin在核酸、蛋白水平均有表达 ,阳性细胞主要分布在胰岛 ,在腺泡周围的外分泌中有少量分布 .结论 :随大鼠胰腺的生长发育 ,核酸、蛋白表达下调 .AIM: To study the expression of nestin in the levels of protein and nucleotide in pancreas of E18.5, newborn and adult rats. METHODS: The mRNA of nestin was amplified by RT PCR, and the number of nestin positive cells and the levels of nestin protein were investigated by immunohistochemistry. RESULTS: Nestin was expressed in E18.5, newborn and adult phases. Nestin positive cells were mostly located in islets and slightly in periacinal exocrines. CONCLUSION: With the development of rat pancreas,the expression of nestin in the levels of nucleotide and protein declines, the percentage of nestin positive cells in islets also decreases.

关 键 词：巢蛋白 胰腺 逆转录-聚合酶链反应 免疫组化 

分 类 号：Q51[生物学—生物化学]