HBV-TR重组腺病毒载体的构建  

Construction of the HBV-TR recombinant adenovirus vector

作　　者：赵亚[1] 宫卫东[1] 刘军[1] 李英辉[1] 丁劲[1] 薛采芳[1] 

机构地区：[1]第四军医大学基础部病原生物学教研室,陕西西安710033

出　　处：《第四军医大学学报》2004年第4期321-324,共4页Journal of the Fourth Military Medical University

基　　金：国家自然科学基金项目 (30 1 0 0 1 57) ;全军医药卫生科研基金项目 (0 1MA1 84) ;陕西省自然科学基金项目 (2 0 0 2C2 2 8)

摘　　要：目的 :构建乙肝病毒靶向核糖核酸酶 (HBVtargetedribonuclease,TR)基因的重组腺病毒载体 .方法 :将HBV TR基因及其对照组基因分别克隆入质粒载体pDC31 6得到pDC31 6 /TR等穿梭质粒 ,然后将所得的穿梭质粒和辅助质粒pBHGlox(delta)E1 ,3Cre以磷酸钙法共转染HEK2 93细胞得到重组腺病毒Ad/TR及对照组重组病毒 ,并以空斑形成实验(PlaqueAssay)测定病毒滴度 .结果 :成功构建了HBV TR重组腺病毒载体Ad/TR及其对照组病毒并获得了高滴度的病毒颗粒 .结论 :HBVAIM: To construct the recombinant adenovirus vector of HBV targeted ribonuclease (HBV TR) gene. METHODS: HBV TR gene and the control groups genes were cloned into plasmid pDC316, respectively, to construct shuttle plasmid pDC316/TR and the control shuttle plasmids. Then the obtained shuttle plasmids were cotransfected with rescue plasmid pBHGlox (delta) E1, 3Cre into HEK293 cells to acquire recombinant adenovirus Ad/TR and control vectors. The recombinant adenoviruses titers were measured by means of plaque assay. RESULTS: Recombinant adenovirus vector of HBV TR gene was successfully constructed and high titers of recombinant adenoviruses were obtained. CONCLUSION: Construction of the HBV TR recombinant adenovirus is successful.

关 键 词：肝炎病毒 乙型 靶向核糖核酸酶 腺病毒载体 

分 类 号：R373.21[医药卫生—病原生物学]