抗Thy-1单克隆抗体能延长小鼠同种皮肤移植的存活时间  

Experimental study on prolongation of survival time of mice skin homograft by anti-Thy-1 monoclonic antibody

作　　者：贠海燕 庞素芳[1] 

机构地区：[1]河南省人民医院整形外科,河南郑州450003

出　　处：《第四军医大学学报》2004年第4期332-334,共3页Journal of the Fourth Military Medical University

摘　　要：目的 :观察局部应用抗小鼠Thy 1单克隆抗体对小鼠皮肤移植存活时间的影响 .方法 :采用间接免疫荧光法检测脾T淋巴细胞亚群的改变及移植皮片的组织学观察作为排斥反应的监测指标 .结果 :抗Thy 1单克隆抗体局部应用可显著延长小鼠同种移植皮片存活时间 ,CD4 + /CD8+ 比值下降和倒置可以作为预测皮肤移植排斥反应发生的有效指标 .结论 :应用抗Thy 1单克隆抗体为大面积烧伤自体皮源不足需要异体皮移植患者延长异体皮肤移植存活时间 ,提供了一个参考依据 .AIM: To study the effects of local application of antilymphocytic monoclinic antibody Thy 1 on the survival time of skin homograft. METHODS: The distribution of splenic T lymphocyte subsets was measured by indirect immunofluorescence assay and the observation of histology was used as the indicator of rejection. RESULTS: The anti Thy 1 monoclonic antibody significantly prolonged the survival time of mice skin homograft ( P <0.01). Decrease (or reverse) of CD4 +/CD8 + ratio could be used as effective indicators of skin homograft rejection. CONCLUSION: Our findings provide some evidence for the application of antihuman T cell monoclinic antibody in prolonging the survival time of skin graft.

关 键 词：皮肤移植 抗Thy-1单克隆抗体 移植物排斥 T淋巴细胞亚群 

分 类 号：R622.1[医药卫生—整形外科]