不同活化系统对CD4^+T细胞凋亡诱导配体表达的影响  

Influence of different stimuli on expression of apoptosis inducing ligands of CD4^+T cells

作　　者：韩卫宁[1] 曹云新[1] 张赟[1] 李琦[1] 刘雪松[1] 金伯泉[1] 

机构地区：[1]第四军医大学基础部免疫学教研室,陕西西安710033

出　　处：《第四军医大学学报》2004年第4期343-346,共4页Journal of the Fourth Military Medical University

基　　金：国家自然科学基金项目 (30 0 0 0 1 50 )

摘　　要：目的 :探讨不同活化系统对CD4 + T细胞凋亡诱导配体在细胞内和膜表面表达的影响 .方法 :分别以PMA、PHA、SEB和单向混合淋巴细胞反应 (MLR)刺激PBMC ,以免疫荧光染色结合三色流式细胞术分析比较刺激前后的CD4 + T细胞中 ,3种主要的凋亡诱导配体TRAIL、FasL和TNF α在细胞内和膜表面表达的变化 .结果 :①FasL在静止CD4 + T细胞表面的表达基本为阴性 ,PMA和PHA上调其表达 ;FasL在静止CD4 + T细胞内有一定程度表达 ,PMA、PHA和MLR下调其在胞内的表达 .②TRAIL在静止CD4 + T细胞表面几乎不表达 ,SEB微弱上调其表达 ;TRAIL在静止CD4 + T细胞胞内有低水平表达 ,SEB和MLR微弱上调其表达 ,PHA则下调其表达 .③TNF α在静止CD4 + T细胞表面几乎不表达 ,PMA和PHA对其表达有一定上调作用 ;TNF α静止CD4 + T细胞胞内有一定水平表达 ,SEB和MLR明显上调其表达 .结论 :FasL、TRAIL和TNF α都是Th细胞重要的效应分子 。AIM: To investigate the influence of different stimuli on the cell membrane and intracellular expression of apoptosis inducing ligands (TRAIL, FasL and TNF α) of CD4 +T cells. METHODS: PBMCs were stimulated by Staphylococcus enterotoxin B (SEB), phorbol myristate acetate (PMA), phytohemagglutinin (PHA) and alloantigen on EB virus transformed B lymphoblastoid cells. The membrane and intracellular expressions of apoptosis inducing ligands were examined by immunofluorescence staining and three color flow cytometry analysis. RESULTS: ① The expression of membrane FasL of the resting CD4 +T cells was almost negative and PMA and PHA could induce FasL expression. The intracellular FasL expressed at a certain level in resting CD4 +T cells and PMA, PHA and alloantigen downregulated the expression. ② The expression of membrane TRAIL on the resting CD4 +T cell was negative and SEB upregulated its expression slightly. TRAIL expressed at a low level in cytoplasm. SEB and alloantigen upregulated its expression slightly whereas PHA downregulated the expression. ③ The expression of membrane TNF α on the resting CD4 +T cell was undetectable and PMA and PHA upregulated the expression. TNF α expressed at a moderate level in cytoplasm and SEB and alloantigen upregulated its expression significantly. CONCLUSION: FasL, TRAIL and TNF α are all important effector molecules of T helper (Th) cells and the expression of these apoptosis inducing ligands both on the membrane and in the cells is regulated by different stimuli.

关 键 词：CD4阳性T淋巴细胞 TRAIL FASL 肿瘤坏死因子 

分 类 号：R392.12[医药卫生—免疫学]