透明质酸在巨噬细胞的分布以及对细胞黏附和迁移的影响  

DISTRIBUTION OF HYALURONAN IN MACROPHAGES ANDEFFECTS OF HYALURONAN ON ADHESION AND MIGRATION OF THE CELLS

作　　者：李奇[1] 王海杰[1] 谭玉珍[1] 王宇鲲[1] 

机构地区：[1]复旦大学上海医学院人体解剖学与组织学胚胎学系,上海200032

出　　处：《解剖学报》2004年第2期152-156,共5页Acta Anatomica Sinica

基　　金：国家教育部博士点基金资助项目 ( 2 0 0 0 0 2 65 0 4)

摘　　要：目的　研究巨噬细胞的透明质酸 (HA)分布以及HA对于巨噬细胞黏附和迁移 ,探讨HA的作用机制。　方法　用聚集蛋白聚糖标记后 ,在光镜和共聚焦激光扫描显微镜下观察巨噬细胞内外的HA分布。　结果　巨噬细胞产生HA。静止细胞的HA多位于细胞表面、细胞膜下和核周。游走细胞内的HA主要分布在伪足、尾部和核周 ,细胞外的HA主要分布于伪足和尾部周围。巨噬细胞表面HA和底物HA增加细胞黏附 ,促进细胞迁移 ,但游离HA降低细胞黏附。　结论　HA在巨噬细胞的分布有特征性。细胞自身产生的HA和底物HA促进巨噬细胞黏附和迁移 ,而游离HA降低巨噬细胞的黏附。Objective To examine location of hyaluronan(HA) in macrophages and effects of HA on adhesion and migration of macrophages and explore regulating mechanism of HA. Methods HA of macrophages was labeled with aggrecan.Location of intracellular and extracellular HA of the cells was viewed using optic microscope and confocal laser scanning microscope.Effects of HA on adhesion and migration of macrophages were examined by adhesion assay and migration assay. Results Macrophage synthesized HA.HA located mainly on the membrane and in premeter and perinuclear area of the quiescent cells.In the moving cells,most of intracellular HA located in pseudopodia,tail and perinuclear area.HA was rich on the surface of the pseudopodia and tail of the cells.HA on the surface of macrophages and HA substratum increased cell adhesion and migration,addition of free HA into the medium decreased cell adhesion.Conclusion There are characteristics of HA distribution in macrophages.HA synthesized by macrophages and HA substratum promoted adhesion and migration of macrophage,while free HA reduces cell adhesion.

关 键 词：透明质酸 巨噬细胞 分布 细胞黏附 迁移 细胞运动 

分 类 号：Q256[生物学—细胞生物学] Q538