一个在小鼠头部特异表达的新基因BSG1 cDNA的克隆及研究  

CLONING AND CHARACTERIZATION OF A NOVEL MOUSE GENE BSG1 cDNA SPECIFICALLY EXPRESSED IN BRAIN

作　　者：吴婷[1,2] 张晶[1,3] 慈宏亮[1,3] 陈微[4] 翟永功[1] 桑建利[5] 左明雪[1] 李亦平[1,3,4] 

机构地区：[1]北京师范大学生物医学研究所 [2]北京师范大学细胞生物学研究所,北京100875 [3]浙江赛尔生物医学研究院,杭州310006 [4]美国哈佛大学Forsyth研究所 [5]北京师范大学细胞生物学研究所

出　　处：《解剖学报》2004年第2期157-160,共4页Acta Anatomica Sinica

基　　金：北京师范大学生物医学研究所科研启动经费资助项目

摘　　要：目的　克隆小鼠头部发育过程中特异表达的新基因并对新基因可能的功能进行初步分析。　方法采用消减差异筛选的方法筛选小鼠头部特异表达的新基因 ,并用生物信息学的方法对克隆到的新基因进行序列分析和蛋白结构域的预测。同时 ,还采用了小鼠胚胎原位杂交、小鼠脑部切片的原位杂交以及鸡胚的原位杂交方法研究BS6 1基因的表达情况。　结果　克隆到 1个与小鼠头部发育相关的新基因BSG1(brainspecificgene 1) ,其GenBank的登录号为AY2 10 4 0 2。该基因包含 1个 2 133bp的完整阅读编码框 ,编码 1个 710aa的C2H2型锌指蛋白。它与人的KIAA0 4 4 1基因在氨基酸水平上有 82 8%的同源性。该基因定位在小鼠的第 10号染色体上 ,含 7个外显子 ,6个内含子。BSG1主要在小鼠胚胎、鸡胚的头部表达 ,并且BSG1在小鼠头部表达的部位局限在海马、齿状回和小脑。　结论　BSG1是 1个可能的转录因子 ,在脑部特异表达。对其研究有助于进一步揭示大脑发育的分子机理。Objective Cloning and characterizing mouse novel genes specifically expressed in brain during the development. Methods Cloning mouse novel genes by subtractive-different screening and characterizing novel gene by bioinformatics methods.At the same time,analyzing the expression pattern of novel gene by whole mount in situ hybridization of mouse embryos and chicken embryos and section hybridization of mouse brain. Results A novel gene BSG1(brain specific gene1) related to development of brain has been cloned and its GenBank accession number is AY210404.It is 2828*!bp long and has a 2133*!bp-long complete reading frame which encodes a C2H2 type zinc finger protein of 710 amino acid residues.It is 82^8% identical to human KIAA0441 gene at the amino acid level.The gene is on mouse chromosome 10 and comprises 7 exons and 6 introns.BSG1 is specifically expressed in mouse brain and chicken brain.In addition, the expression in mouse brain is specifically restricted to hippocampus,dentate gyrus and cerebellum.Conclusion BSG1 is a putative transcription factor and specifically expressed in brain.Study of BSG1 will contribute to reveal the molecular mechanism of the development of brain.

关 键 词：小鼠 特异表达 新基因 BSG1cDNA 克隆 生物信息学 锌指结构域 

分 类 号：Q786[生物学—分子生物学]