胃腔内促性腺激素释放激素类似物对大鼠消化道胃泌素细胞功能的影响  

THE EFFECT OF GnRH-A ON THE FUNCTION OF GASTRIN CELLS IN DIGESTIVE TRACT OF RATS

作　　者：刘晓宁[1] 朱晓东[1] 赵洁[1] 黄威权[1] 

机构地区：[1]第四军医大学组织学与胚胎学教研室,西安710032

出　　处：《解剖学报》2004年第2期190-193,共4页Acta Anatomica Sinica

基　　金：国家自然科学基金资助项目 ( 3 9770 3 88)

摘　　要：目的　研究胃腔内促性腺激素释放激素 (GnRH)类似物对大鼠消化道胃泌素免疫阳性细胞及血液、胃液中胃泌素含量的影响。　方法　应用免疫组织化学和酶联免疫分析 (ELISA)方法分别检测大鼠消化道内胃泌素阳性细胞的密度及血液、胃液中胃泌素的含量。　结果　胃腔注射GnRH类似物后 ,胃壁内单位面积胃泌素阳性细胞数为 19 6 0± 3 6 3,与对照组 10 30± 2 4 1相比 (P <0 0 1) ;而十二指肠阳性细胞数为 18 0 0± 2 31,与对照组 9 0 0± 2 0 5相比 (P <0 0 1)。血液中胃泌素ELISA检测的A值为 0 5 5± 0 0 83,与对照组 0 2 3± 0 0 39相比 (P <0 0 1) ;胃液中为 0 5 2± 0 0 83,与对照组 0 30± 0 0 31相比 (P <0 0 1)。　结论　外分泌的GnRH对大鼠消化道中胃泌素的合成与分泌均起显著的促进作用。Objective To investigate the effect of GnRH-A in gastric cavity on function of gastrin clls in digestive tract of rats. Methods The immunohistochemical ABC method and enzyme-link immunoassay were used in the experiment. Results The densities of the gastrin immunoreactivity cells in the stomach and small instestin of the experiment groups were 19.60±3.63 and 18.00±2.31 respectively, those in stomach was 10.30±2.41 while in small intestin was 9.00±2.05 of control groups respectively (P<0.01). The contents of gastrin in circulation blood and gastric liquid of the experiment groups were 0.55±0.083 and 0.52±0.083 respectively, and those in circulation blood was 0.23±0.039 whlie in gastric liquid was 0.30±0.031 in control groups respectively(P<0.01).Conclusion The exocrine GnRH in the stomach cavity significantly stimulated synthesis and secretion of gastrin cell in digestive tract of rats.

关 键 词：促性腺激素释放激素类似物 大鼠 消化道胃泌素 细胞功能 胃泌素 

分 类 号：R333.2[医药卫生—人体生理学]