神经丝的完整性对线状溶酶体形状与分布的影响  

THE EFFECT OF THE INTEGRITY OF NEUROFILAMENT ON THE SHAPE AND DISTRIBUTION OF NEMATOLYSOSOME

作　　者：黄瑛[1] 柳达[2] 石玉秀[3] 

机构地区：[1]温州医学院附属二院超声科,温州325027 [2]温州医学院附属二院骨科,温州325027 [3]中国医科大学组织学胚胎学教研室,沈阳110001

出　　处：《解剖学报》2004年第2期198-201,共4页Acta Anatomica Sinica

基　　金：国家自然科学基金资助项目 ( 3 9970 3 83 )

摘　　要：目的　观察神经丝的完整性是否对线状溶酶体的形状与分布有影响。　方法　采用SABC单克隆抗体免疫组织化学法、免疫电镜及酶组织化学、电镜酶组织化学技术观察钒酸盐处理后培养的神经元内神经丝蛋白NF H及线状溶酶体的改变。　结果　当神经丝的完整性被破坏时 ,神经丝蛋白向核周聚集 ,并伴随线状溶酶体亦向核周聚集并形状改变。　结论　钒酸盐通过多途径作用 ,使神经丝过度磷酸化 ,失去装配能力 ,当神经丝结构被破坏时 ,线状溶酶体的形状及分布都发生变化。线状溶酶体的形状与分布对神经丝的完整性有依赖作用。Objective To confirm that if the integrality of NF has effect on the shape and distribution of NLY. Methods using SABC monocolon antibody immunohistochemistry,electron microscopy,immunocytochemistry, and enzymocytochemistry to observe the disorder of NF-H and the changes of distribution and shape of NLY after vanadate treated. Results When the integrality of NF was damaged, the proteins of NF gathered towards nuclear accompany with the similar movements of NLY, meanwhile the shape of NLY also changed into round from thread-like shape.Conclusion Through different ways we used, vanadate can over-phosphate NF proteins and destroy the assemble ability. Whether NF has a complete structure is important to the shape and distribution of NLY, which will be changed when the structure of NF is disorganized.

关 键 词：神经丝 完整性 线状溶酶体 形状 分布 免疫组织化学 神经细胞培养 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]