硫芥中毒犬外周血IL-2、IL-6含量变化和淋巴细胞DNA损伤  

Sulfur mustard-induced changes of IL-2 and IL-6 concentrations and lymphocyte DNA damage in dog peripheral blood

作　　者：林海[1] 陈志龙[2] 董兆君[1] 王仕丽[1] 

机构地区：[1]第三军医大学预防医学系军事毒理学教研室,重庆400038 [2]第二军医大学海医系,上海200433

出　　处：《第三军医大学学报》2003年第12期1038-1041,共4页Journal of Third Military Medical University

基　　金：全军"十五"重点课题资助项目 ( 0 1L0 54 )

摘　　要：目的　研究硫芥中毒犬外周血IL 2、IL 6变化和淋巴细胞DNA损伤的规律。方法　重庆家犬硫芥中毒 ( 16mg kg ,sc)。中毒后不同时相点取血 ,分离血清和淋巴细胞。采用放射免疫方法测定血清中IL 2、IL 6含量 ,以单细胞凝胶电泳技术测淋巴细胞DNA损伤程度。结果　中毒后 4h血清IL 2、IL 6含量开始下降 ,72h达最低值 ,12 0h开始恢复。单细胞电泳检测结果提示 ,硫芥导致明显的淋巴细胞彗星现象。受损细胞率、淋巴细胞DNA片段迁徙度于中毒 4h即开始升高 ,2 4～ 72h持续升高。结论　硫芥中毒导致发生早且持续时间较长的犬外周血IL 2、IL 6含量下降和淋巴细胞DNA损伤。Objective To study the changes of IL 2 and IL 6 levels and lymphocyte DNA damage in peripheral blood of dog intoxicated by sulfur mustard. Methods Chongqing dogs were subcutaneously injected with sulfur mustard at the dose of 16 mg/kg. Lymphocytes and serum were isolated from dog peripheral blood at different time points as designed. The IL 2 and IL 6 levels in blood serum were measured by radioimmunoassay and lymphocyte DNA damage represented as comet tail length was determined by single cell gel electrophoresis (SCGE). Results Four hours after administration of sulfur mustard, the serum IL 2 and IL 6 levels dogs injected with sulfur mustard decreased and reached the lowest level at 72 h. A significant recovery was found at the time point of 120 h. SCG revealed sulfur mustard induced DNA fragmentation (comet) in dog peripheral lymphocytes. The rate of the damaged lymphocytes and the degree of DNA fragment migration began to increase at 4 h and progressively increased from 24 h to 72 h after sulfur mustard treatment. Conclusion Sulfur mustard intoxication induces early and long lasting decreases in serum IL 2 and IL 6 levels and lymphocyte DNA fragmentation in dog peripheral blood.

关 键 词：硫芥 白介素 单细胞电泳 

分 类 号：R446.62[医药卫生—诊断学] R446.9[医药卫生—临床医学]