大鼠烧伤复合内毒素血症致肝脏细胞凋亡实验研究  

Liver cell apoptosis due to burn injury combined with endotoxemia

作　　者：孙素群[1] 许霖水[1] 何凤田[1] 

机构地区：[1]第三军医大学基础医学部生物化学与分子生物学教研室,重庆400038

出　　处：《第三军医大学学报》2003年第12期1062-1064,共3页Journal of Third Military Medical University

摘　　要：目的　研究大鼠烧伤复合内毒素血症早期肝细胞的凋亡规律 ,并初步探讨其机制。方法　烧注组以大鼠体表面积 2 0 %Ⅲ度烧伤复合内毒素血症为模型 ,与正常对照、单烧组、单注组比较 ,观察其肝脏病理变化、TUNEL检测、DNA凝胶电泳的改变 ,并测量血浆MDA浓度和SOD活性。结果　烧注组凋亡细胞的数量明显比后两组多且发生时间早 ,于伤后 6h达到峰值。血浆MDA含量升高趋势和SOD活性降低趋势与肝细胞凋亡发生相一致。结论　烧伤复合内毒素血症大鼠早期肝细胞损伤以凋亡为主 ,脂质过氧化是其发生机制之一。Objective To observe the pathological changes of liver cells in the early periods of burn injury combined with endotoxemia and to explore its mechanisms preliminarily. Methods Rats were inflicted with 20% TBSA Ⅲ degree burn combined with intraperitoneal injection of lipopolysaccharide (burn injury combined with endotoxemia, combined group) or simply burned or simply injected. Each group included 25 rats, with 5 rats left for control. Rats in the former 3 groups were sacrificed and sampled at 1, 3, 6, 12 and 24 h after injury, 5 rats for each time point. The results were observed by HE staining, TUNEL and DNA gel electrophoresis, and the concentrations of plasma MDA and the activity of plasma SOD were assessed and analyzed. Results Apoptosis of liver cells was found in each of the 3 groups but its regularity was different from one another. Much more apoptotic cells in the combined group were found than the other 2 groups and apoptosis happened earlier with its maximum at 6 h postinjury and declined thereafter. Much less apoptotic cells were found in the simply burned group and reached the maximum at 12 h postburn. There were only a few apoptotic cells at 6 h postinjection in the simply injected group in which there were the fewest apoptotic cells in the three groups. The concentration of plasma MDA and the activity of SOD always moved in the opposite way at the same time. In the combined group, the maximum of MDA concentration and the minimum of SOD activity happened at the same time point, which was just before the one of the maximum of liver cell apoptosis. Conclusion During the early period of burn injury combined with endotoxemia, rat liver cells die mainly in the apoptotic way, with lots of apoptotic cells at 6 h and 12 h postinjury, combined with necrosis at 24 h. Lipid peroxidation may partly account for the apoptosis of liver cells.

关 键 词：内毒素血症 肝脏 凋亡 脂质过氧化 

分 类 号：R644[医药卫生—外科学] R329.28[医药卫生—临床医学]