低体质量儿童外周血干细胞采集  

Collection of stem cells from peripheral blood in low body weight children

作　　者：段慧玲[1] 滕本秀[1] 

机构地区：[1]第三军医大学附属新桥医院输血科,重庆400037

出　　处：《第三军医大学学报》2003年第12期1101-1103,共3页Journal of Third Military Medical University

摘　　要：目的　探索一套安全、有效的低体质量 (体质量 <2 0kg)小儿外周血干细胞采集方法。方法　 5名体质量 <2 0kg的患儿 ,共 15次采集外周血干细胞。在采集前 1d行股静脉穿刺 ,留置一儿童双腔管。使用儿童分离槽和收集槽进行收集。并在初始化结束后 ,用经射线照射过的异体血液预先运转分离机 ,以保证儿童体内的血液平衡和红细胞压积的稳定 ;在分离过程中 ,严格保持ACD 全血的比例为 1∶11～ 1∶13 ;每次处理总血量为儿童全身总血容量的 2～ 4倍 ( 2 70 0～5 85 0ml)。结果　 5名患儿每次均采集到足够的MNC和CD3 4+ 细胞 ,达到外周血干细胞移植的阈值要求 ,保证了PBSCT的成功。结论　使用本方法能安全、有效的进行低体质量小儿外周血干细胞的采集。Objective To find a safe and effective way for the collection of stem cells from peripheral blood in low body weight children. Methods Peripheral blood stem cells were collected altogether 15 times in 5 children with body weight lower than 20 kg. One day before the collection, a double cavity tube specially made for children was inserted into femoral vein and remained there for future use. Peripheral blood stem cells were separated by the separation vessel specially designed for children and collected in the collecting tank for children. After initialization, alien blood irradiated by X ray was infused in and moved through the separation machine to keep the blood balanced and hematocrit stable in the body of children. During the separation, anticoagulant/whole blood was kept between 1∶11 and 1∶13. The volume of blood processed every time was 2-4 times of total volume of child blood(2 700-5 850 ml). Results Mononuclear cells and CD34 + cells were successfully collected each time in all 5 children, enough for the transplantation of peripheral blood stem cells. Conclusion The way we developed to collect peripheral blood stem cells in children with low body weight is safe and effective.

关 键 词：儿童 采集/移植 外周血干细胞 恶性血液病 

分 类 号：R457[医药卫生—治疗学] R733[医药卫生—临床医学]