吸氧对慢性缺氧高二氧化碳大鼠肺血管L-精氨酸转运的影响  

Effect of oxygen on L-arginine transport in pulmonary arteries in rats chronically exposed to hypoxia-hypercapnia

作　　者：龚永生[1] 范小芳[1] 刘道侯[1] 胡良冈[1] 李继武[1] 唐朝枢[2] 庞永正[2] 蒋仲荪[1] 

机构地区：[1]温州医学院肺心病研究室,325003 [2]北京大学医学部心血管病研究所

出　　处：《中华结核和呼吸杂志》2003年第7期403-406,共4页Chinese Journal of Tuberculosis and Respiratory Diseases

基　　金：浙江省卫生厅基金资助项目 (sws 960 0 5 );温州市科技计划项目资助 (S2 0 0 1A2 4)

摘　　要：目的　探讨慢性缺氧 (O2 )高二氧化碳 (CO2 )对大鼠肺动脉L 精氨酸 (L Arg)转运的影响与吸氧的作用。方法　将 4 0只大鼠以随机区组设计法分成 4组 ,用 0 2mmol/L和 5 0mmol/L [3 H] Arg将肺动脉孵育 ,测定其对 [3 H] L Arg的摄取率、一氧化氮合酶 (NOS)活性与一氧化氮 (NO2 /NO3)含量。每组 1 0只。正常对照组 (A组 )、慢性缺O2 4周后伴高CO2 4周组 (B组 )、慢性缺O2 4周伴高CO24周后吸空气 4周组 (C组 )及慢性缺O2 4周伴高CO2 4周后吸O2 4周组 (D组 )。结果　 (1 )肺动脉平均压 (mPAP)、右心室 (RV)和左心室 +室间隔 (LV +S)重量比值 (RV/LV +S) :B组 (33% )与A组(35 % )比较差异有显著性 (P均 <0 0 1 ) ;D组 (2 4 % )与C组 (2 4 % )比较 ,差异也有显著性 (P均 <0 0 5 )。 (2 )离体孵育的肺动脉摄取低浓度 (0 2mmol/L)和高浓度 (5 0mmol/L) [3 H] L Arg比较 :B组分别为 [(3 0 4± 0 1 6 ) μmol·g-1 min-1 ]、[(8 1 2± 0 1 4 ) μmol·g-1 ·min-1 ],A组分别为 [(4 6 2± 0 5 5 )μmol·g-1 ·min-1 ]、[(1 1 2 4± 1 0 2 ) μmol·g-1 ·min-1 ],A、B两组比较差异有显著性 (P均 <0 0 1 ) ;而D组分别为 [(4 30± 0 1 8) μmol·g-1 ·min-1 ]、[(1 2 31± 0 6 5 ) μmol·g-1Objective To investigate the effect of chronic hypoxia hypercapnia and oxygen inhalation on L arginine transport in rat pulmonary artery Methods Forty male Sprague Dawley rats were randomly divided into four groups: normal control group (A), the group of 4 week hypoxia hypercapnia after 4 week hypoxia (B), the group of 4 week oxygen inhalation (D) 4 week exposure to air (C) after 4 week hypoxia and 4 week hypoxia hypercapnia Changes in pulmonary artery L arginine (L Arg) transport, nitric oxide synthase (NOS) activity, plasma nitrite (NO 2 /NO 3 ) concentration and L Arg level in rats were measured Results (1) The mean pulmonary artery pressure (mPAP) and weight ratio of right ventricle to left ventricle plus septum (RV/LV+S) of B group were higher than those of A group (33% and 35%, respectively, P< 0 01), with the D group lower than C group (24% and 24%, respectively, P< 0 05) (2) At low (0 2 mmol/L) or high (5 0 mmol/L) concentration of L Arg,the velocity of L Arg transport in B group was lower than that in A group [(3 04±0 16 vs 4 62±0 55) μmol·g -1 ·min -1 and (8 12±0 14 vs 11 24±1 02) μmol·g -1 ·min -1 , respectively, P< 0 01], and in D group higher than in C group [(4 30±0 18 vs 3 80±0 21)μmol·g -1 ·min -1 and [(12 31±0 65 vs 10 04±1 28) μmol·g -1 ·min -1 , respectively, P< 0 01] (3) The activity of pulmonary artery tNOS and iNOS in B group was significantly higher than that in C group [(3 82±0 42 vs 2 59±0 22) μnmol·g -1 ·min -1 and (3 07±0 30 vs 1 62±0 10) nmol·g -1 ·min -1 respectively, P< 0 01], but cNOS in B group was lower than that in NC group [(0 75±0 16 vs 0 93±0 10) nmol·g -1 ·min -1 , P< 0 01] The activity of tNOS and cNOS was not significantly different between D group and C group ( P> 0 05); However,iNOS was lower in D group than in C group [(1 97±0 18 vs 2 25±0 17) nmo

关 键 词：吸氧 慢性缺氧 高二氧化碳 大鼠 肺血管 L-精氨酸 

分 类 号：R543.2[医药卫生—心血管疾病]