VEGF对人血单核细胞TIMPs表达的影响  

Effect of VEGF on Expression of TIMPs in Human Monocytes

作　　者：王长谦[1] 汤大鸣[1] 黄定九[1] 王彬尧[1] 王利民[1] 宋伟[1] 

机构地区：[1]上海第二医科大学仁济医院心内科,上海200001

出　　处：《上海第二医科大学学报》2003年第3期200-202,209,共4页Acta Universitatis Medicinalis Secondae Shanghai

基　　金：上海市青年科技启明星计划资助项目(98QB14014)

摘　　要：目的 探讨血管内皮生长因子(VEGF)对人单核细胞源巨噬细胞(HMDM)组织基质金属蛋白酶抑制物(TIMPs)的基因和蛋白表达的影响。 方法 分别应用RT-PCR和Western blot检测TIMP(TIMP-1和TIMP-2)基因和蛋白表达。结果 VEGF可明显抑制HMDM TIMP-1 mRNA、TIMP-2 mRNA及其蛋白表达。 结论 VEGF可抑制HMDM TIMP-1 mRNA、TIMP-2 mRNA及其蛋白的表达,可能间接增强基质金属蛋白酶(MMPs)活性,因而有可能促进动脉粥样硬化斑块内基质降解,进而引起粥样硬化斑块破裂。Objective To observe the effect of VEGF on the expressions of tissue inhibitors of metalloproteinase (TIMPs). Methods The gene and protein expressions of TIMPs (TIMP-1 and TIMP - 2) were respectively detected by RT - PCR and Western blot. Results VEGF can obviously decrease the expressions of TIMP - 1 and TIMP - 2 in human monocytes. Conclusion VEGF can decrease the expressions of TIMP - 1 and TIMP - 2 in human monocytes, may be helpful to promote the breakdown of the matrix in the atherosclerotic lesions and enhance their vulnerability.

关 键 词：血管内皮生长因子 单核细胞 巨噬细胞 组织基质金属蛋白酶抑制物 动脉粥样硬化 细胞培养 

分 类 号：R543.5[医药卫生—心血管疾病]