双歧杆菌纯化黏附素对ETEC和EPEC黏附肠上皮细胞的竞争抑制作用  

Competitive inhibition of adherence of ETEC and EPEC to intestinal epithelial cells by purified adhesin of bifidobacterium adolescentis 1027

作　　者：钟世顺[1] 张振书[1] 赖卓胜[1] 王群英[1] 杨玉捷[1] 王继德[1] 

机构地区：[1]第一军医大学南方医院,广州510515

出　　处：《解放军医学杂志》2004年第3期243-245,共3页Medical Journal of Chinese People's Liberation Army

基　　金：广东省自然科学基金资助课题 (编号 0 1 0 62 1 )

摘　　要：目的　观察双歧杆菌分泌型黏附素对产毒性大肠杆菌 (ETEC)和致病性大肠杆菌 (ETEC)黏附肠上皮Lovo细胞的竞争抑制作用。方法　采用黏附试验计数肠上皮Lovo细胞黏附的细菌数并比较其结果。结果　除黏附素浓度为 1μg/ml和 5 μg/ml时不能明显抑制ETEC和EPEC对Lovo细胞的黏附外 ,10 μg/ml、2 0 μg/ml、30 μg/ml浓度组均能明显抑制ETEC和EPEC对Lovo细胞的黏附 ,且随浓度的逐渐增加 ,这种抑制作用也逐渐增强。Objective To observe competitive inhibition of adherence of enterotoxigenic Escherichia coli (ETEC) and enteropathogenic Escherichia coli (EPEC) to intestinal epithelial cells by purified adhesin derived from bifidobacterium adolescentis 1027. Methods The number of bacteria adherent to intestinal epithelial cell line Lovo was counted and the results were analyzed by comparison. Results The purified adhesin in the concentration of 10μg/ml, 20μg/ml and 30μg/ml could significantly inhibit the adhesion of ETEC and EPEC to intestinal epithelial cell line Lovo. The higher the concentration of adhesin the higher inhibition rate was observed. Concentrations lower than 10μg/ml had no such effect. Conclusion The purified adhesin of bifidobacterium adolescentis 1027 could inhibit the adhesion of ETEC and EPEC to intestinal epithelial cell line Lovo, and the effect was dose-dependent.

关 键 词：二裂菌属 黏附素 肠产毒性大肠杆菌 肠致病性大肠杆菌 细菌黏附 肠上皮细胞 

分 类 号：R378.21[医药卫生—病原生物学]