Effect of decoy-oligodeoxynucleotides on expression of inflammation mediators in pMΦ cells from rats  

作　　者：国华 梁华平 吕凤林 徐祥 安兵 郝天智 王付龙 杨文军 

机构地区：[1]Research Institute of Surgery, Daping Hospital, Third Military Medical University

出　　处：《Chinese Journal of Traumatology》2003年第6期363-369,共7页中华创伤杂志（英文版）

基　　金：ThisstudywassupportedbytheNationalNaturalScienceFoundationofChina (No .30 170 36 7),theTenth FiveYearPlanofMilitaryCommand (No .0 1MB113)andMajorBasicResearchDevelopmentProgramofChina (No .G19990 5 4 2 0 3)

摘　　要：Objective: To study the effect of decoy-oligodeoxynucleotides (decoy-ODNs) in dumbbell shape with the oligodeoxynucleotide sequence similar to nuclear factor kappa B (NF-κB) cis-elements on expression of inflammation mediators in pMΦ cells from rats. Methods: With carriers of cationic liposomes, decoy-ODNs were transfected into pMΦ cells of rats. Then the inhibiting effects of the decoy-ODNs on tumor necrosis factor α (TNFα), interleukin-6 (IL-6) and IL-10 were analyzed. Results: Decoy-ODNs could decrease the expression of TNFα and IL-6 in dose-dependent fashion but had weaker inhibiting effect on IL-10. Conclusions: Decoy-ODNs targeting NF-κB can decrease the expression of inflammatory mediators in pMΦ cells from rats.

关 键 词：Tumor necrosis factor Inflammation mediators Nuclear factor kappa B Decoy-oligodeoxynucleotides 

分 类 号：R602[医药卫生—外科学]