mPEG修饰淋巴细胞HLA-A_2抗原的研究  

Methoxypolyethylene glycol-modified HLA-A_2 antigen on lymphocytes

作　　者：张印则[1] 李伟[2] 周华友[1] 单小燕[2] 兰炯采[1] 章扬培[3] 张志新[2] 

机构地区：[1]第一军医大学南方医院输血科,广东广州510515 [2]北京红十字血液中心,北京100088 [3]军事医学科学院野战输血研究所,北京100850

出　　处：《第一军医大学学报》2003年第6期557-560,共4页Journal of First Military Medical University

基　　金：国家高技术发展规划863项目(2001AA261161)~~

摘　　要：目的用甲氧基-聚乙二醇(mPEG)修饰淋巴细胞表面HLA-A2抗原,阻断它与相应抗体的特异性结合。方法在不同温度、不同pH的磷酸缓冲液(PBS)中分别使用终浓度为12 mmol/L不同种类的mPEG对淋巴细胞表面HLA-A2抗原进行修饰。结果4 ℃及室温对淋巴细胞表面HLA-A2抗原修饰效果无明显影响,30 ℃及37 ℃条件下不利于修饰;高浓度mPEG在碱性环境中对HLA-A2抗原的修饰效果较好,且不同mPEG对修饰效果亦有影响。结论室温下,在pH7.4的PBS介质中mPEG-BTC(benzotriazole carbonate)对淋巴细胞表面HLA-A2抗原的修饰效果最好,其次为mPEG-丙酸-琥珀酰亚胺,mPEG-马来酰亚胺无修饰能力。Objective To modify the HLA-A 2 antigen on the lymphocytes with methoxypolyethylene glycol (mPEG) so as to block the specific binding site for antibody. Method Different types of mPEG (all with final concentration of 12 mmol/L) were used at different temperatures in PBS with varied pH values for the modification of the HLA-A 2 antigen. Result The modification of the antigen was not obviously affected when it was carried out at 4 ℃or room temperature, but higher tem- peratures of 30 and 37 ℃significantly hampered the modification. Better antigen modification was observed with high-concentration mPEG in basic PBS, denpending also on the type of mPEGs used for this purpose. Conclusion The specific HLA-A 2 binding on the lymphocytes is completely blocked by benzotriazole carbonate-mPEG(mPEG-BTC), which is superior to N-hydroxysuccinimidyl ester of mPEG(mPEG-SPA). Maleimide-mPEG(mPEG-MAL) is incapable of blocking the HLA-A 2 ligand-binding site with antibody.

关 键 词：MPEG 修饰 淋巴细胞 HLA-A2抗原 淋巴细胞 

分 类 号：R392[医药卫生—免疫学]