人发角蛋白人工腱诱导自体腱形成中胶原原纤维的形态学变化  

Morphological changes of collagen fibrils during the formation of autogenous tendon induced by human hair keratin artificial tendon

作　　者：王启伟[1] 朴英杰[1] 王万山[1] 路艳蒙[1] 傅文玉[1] 

机构地区：[1]第一军医大学组织胚胎学教研室,广东广州510515

出　　处：《第一军医大学学报》2003年第6期569-571,共3页Journal of First Military Medical University

基　　金：国家自然科学基金(930314)~~

摘　　要：目的观察人发角蛋白(HHK)人工腱诱导自体腱形成过程中胶原原纤维形成的形态学变化。方法制备肌腱损伤动物模型,植入HHK人工腱,于术后3、6、9、12、16周取出,进行光镜及电镜观察。结果自体腱形成过程中,先后有一级、二级和三级胶原原纤维形成。结论HHK人工腱诱导自体腱形成过程中,人工腱植入区肌腱断端和腱膜下的腱细胞去分化,分泌大量的胶原蛋白,并在细胞外生成一级胶原原纤维,再逐级融合形成二级和三级胶原原纤维,最终多数腱细胞胶原化而消失。Objective To observe the morphological changes of collagen fibrils during the formation of autogenous tendon induced by human hair keratin (HHK) artificial tendon. Methods Rabbit models of injured tendon were established in which implantation of HHK artificial tendon was performed to observe the formation of autogenous tendon under light microscope and electron microscope at 3, 6, 9, 12 and 16 weeks after HHK implantation. Results and Conclusion During autogenous tendon formation induced by HHK artificial tendon, the tendon cells of the impaired end of the tendon and beneath the tendon membrane dedifferentiate and are capable of collagen secretion, followed by the formation of typesⅠ,ⅡandⅢcollagen fibrils, and eventually, the majority of the tendon cells disappear with the collagenization of the tendon.

关 键 词：人发角蛋白 人工腱 诱导 自体腱形成 胶原原纤维 形态学 

分 类 号：R318.17[医药卫生—生物医学工程]