重庆地区人群亚甲基四氢叶酸还原酶基因多态性与结直肠癌易感性关系研究  

MTHFR polymorphisms and colorectal cancer susceptibility in Chongqing people

作　　者：黄萍[1] 周紫垣[1] 马恒太[1] 刘晋祎[1] 周燕虹[1] 曹佳[1] 葛海燕[2] 余佩武[3] Toshiro Takezaki 

机构地区：[1]第三军医大学军事预防医学院卫生毒理学教研室,重庆400038 [2]第三军医大学附属新桥医院普通外科,重庆400037 [3]第三军医大学附属西南医院普通外科,重庆400038 [4]Division of Epidemiology and Prevention,Aichi Cancer Center Research Institute,Nagoya,Japan

出　　处：《第三军医大学学报》2003年第19期1710-1713,共4页Journal of Third Military Medical University

基　　金：日本文部省优先资助领域C科研基金资助项目 ( 12 2 182 4 2 )

摘　　要：目的　探讨重庆人群亚甲基四氢叶酸还原酶 (Methylenetetrahdrofolatereducatase ,MTHFR)基因多态性与结直肠癌的关系 ,研究叶酸、MTHFR基因多态性以及结直肠癌发生的交互作用。方法　①用聚合酶链反应限制性片段多态性(PCR RFLP)技术 ,检测 82例结直肠癌患者和 82例正常对照的MTHFR基因多态性。②使用放免方法检测病例及对照血浆中的叶酸含量。结果　①病例组叶酸水平显著低于对照组。②结直肠癌患者与对照MTHFR突变基因型率分别为 5 6 1%和 5 1 3 %,无统计学差异。结论　叶酸水平与结直肠癌的发生显著相关 ,是结直肠癌发病的保护因素。Objective To explore the relationship between methylenetetrahydrofolate reductase(MTHFR) polymorphisms and colorectal cancer susceptibility and to observe the interaction of MTHFR polymorphism, folate concentration and colorectal susceptibility Methods Polymerase chain reaction restriction fragment length polymorphism (PCR RFLP) technique was used to analyze the genotype of MTHFR in 82 cases of colorectal cancer and 82 controls Folate levels in sera of the cases and controls were measured by radioimmunoassay(RIA) Results The folate level in the case group was significantly lower than that in the control No significant difference in the frequency of MTHFR variant genotype was found between the cases and the controls(56 1% vs 51 3%) Subjects with low folate level were at a significantly higher risk of developing colorectal cancer Conclusion Folate level is significantly associated with colorectal susceptibility Folate is a colorectal cancer protective factor In this study, MTHFR gene polymorphism might not be related with colorectal cancer

关 键 词：结直肠癌 亚甲基四氢叶酸还原酶 叶酸 限制性片段长度多态性 

分 类 号：R181.3[医药卫生—流行病学] R735.3[医药卫生—公共卫生与预防医学]