当归多糖对大鼠Kupffer细胞免疫功能的激活作用  

Activation of the immunologic function of rat Kupffer cells by the polysaccharides of Angelica sinensis

作　　者：王君[1] 夏雪雁[1] 彭仁琇[1] 陈效[1] 

机构地区：[1]武汉大学医学院药理学系

出　　处：《药学学报》2004年第3期168-171,共4页Acta Pharmaceutica Sinica

摘　　要：目的　观察当归多糖 (ASP)对大鼠Kupffer细胞免疫功能的影响。方法　ASP体外作用于正常Kupffer细胞 ,以吞噬中性红A540 、分泌NO和TNF α量评价其免疫功能 ;以LDH漏出量 ,评价ASP对细胞的直接损害情况。大鼠igASP后 ,检测Kupffer细胞上述指标及胞内酸性磷酸酶 (ACP)活性 ;以sALT和sGST为肝毒性指标。结果　ASP增强Kupffer细胞对中性红吞噬作用、增加ACP活性及NO和TNF α的产生。ASP在体外不增加肝实质细胞LDH漏出 ,而体内使sGST升高。结论　适当剂量ASP可激活Kupffer细胞免疫功能。大剂量ASP出现的轻度肝功能改变可能与NO和TNF α等免疫因子过度分泌间接有关。Aim To observe the influence of polysaccharides of Angelica sinensis (ASP) on the immunologic function of rat Kupffer cells. Methods Normal rat Kupffer cells were treated with ASP in vitro . Absorbance at 540 nm ( A 540 ) of neutral red absorption and supernatant NO, TNF-α in the cells were measured to evaluate the immunologic function of Kupffer cells; LDH leakage was measured to estimate the severity of cellular damage; Rats were given ASP 0.025, 0.1, 0.25 and 1.0 g·kg -1 ig (qd×7 d) in vivo . The above indices and ACP of Kupffer cells were measured, sGST and sALT activity were detected as indices of hepatotoxicity. Results ASP markedly enhanced the phagocytic activity, ACP and supernatant NO, TNF-α of Kupffer cells both in vitro and in vivo . The increase of sGST was observed after administration of ASP 1.0 g·kg -1 , but the LDH leakage of the hepatocytes was not increased in vitro . Conclusion ASP with suitable dose could activate the function of Kupffer cells. Slight liver injury was caused by ASP 1.0 g·kg -1 in vivo, which was likely caused by factors, such as NO, TNF-α, indirectly.

关 键 词：当归多糖 大鼠 KUPFFER细胞 免疫功能 激活作用 

分 类 号：R967[医药卫生—药理学] R282.71[医药卫生—药学]