银杏总内酯对衰老模型小鼠的作用  

Anti-aging action of the total lactones of ginkgo on aging mice

作　　者：董六一[1] 范丽[1] 李桂芳[1] 郭岩[1] 潘见[2] 陈志武[1] 

机构地区：[1]安徽医科大学药理教研室,安徽合肥230032 [2]合肥工业大学生物机电所,安徽合肥230061

出　　处：《药学学报》2004年第3期176-179,共4页Acta Pharmaceutica Sinica

基　　金：安徽省教委重点科研项目 (98j10 80 2 )

摘　　要：目的　研究银杏总内酯 (totallactonesofginkgo ,TLG)对衰老模型小鼠的影响 ,探讨TLG的抗衰老作用机制。方法　以D 半乳糖衰老模型小鼠和自然衰老模型小鼠为实验对象 ,以D 半乳糖衰老模型小鼠心、肝组织中脂褐素(LF)的含量及肝中羟脯氨酸 (Hyp)的含量 ,脑组织中MAO ,GSH Px的活性和NO的含量以及自然衰老模型小鼠脑组织凋亡细胞数作为观察指标。结果　TLG可减少D 半乳糖小鼠肝脏及心肌组织中LF的含量 ,升高肝脏组织中的Hyp的含量 ,并可提高脑组织中GSH Px的活性 ,降低脑组织中NO的含量和MAO的活性 ;能明显减少自然衰老模型小鼠脑神经细胞凋亡的数目。结论　TLG有明显的延缓衰老作用 ,其作用机理可能与抗氧化、抑制NO生成及细胞凋亡有关。Aim To investigate the effects of total lactones of ginkgo on aging by using D -galactose induced aging mice and natural aging mice. Methods By using D -galactose induced aging mice, to detect the LF content in heart and liver, the Hyp content in liver, the MAO, GSH-Px activities and the NO content in cerebrum. The apoptosis of cerebral cell was determined by terminal deoxy-nucleotidyl transforase-mediated dUTP-digoxigenin nick end-labeling (Tunel) in natural aging mice. Results TLG was shown to increase the GSH-Px activities, reduce the NO content and decrease the MAO activity in cerebrum. Meanwhile, TLG was found to reduce the LF content in liver and heart and raise the Hyp content in liver. TLG was shown to inhibit apoptosis of cerebral cell and decrease the number of apoptotic cells in the brain. Conclusion TLG possesses effect on antiaging via attenuating lipid peroxidation and NO and apoptosis of cerebral cells.

关 键 词：银杏总内酯 衰老模型 小鼠 D-半乳糖 细胞凋亡 抗衰老作用 

分 类 号：R286.7[医药卫生—中药学]