蛋白质及纤维素衍生物手性固定相分离盐酸西替利嗪对映体  

Study on the enantiomer separation of cetirizine dihydrochloride using proteinate-and amylose-based chiral stationary phase

作　　者：张哲峰[1] 杨更亮[1] 梁贵键[2] 周宇[1] 陈义[3] 

机构地区：[1]河北大学化学与环境学院 [2]空军总医院,北京100850 [3]中国科学院化学研究所分子科学中心,北京100080

出　　处：《药学学报》2004年第3期204-207,共4页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金资助项目(2 0 0 75 0 0 5;2 92 0 5 0 3 7);河北省自然科学基金资助项目(2 0 0 0 77)

摘　　要：目的　研究盐酸西替利嗪对映体在蛋白质及纤维素衍生物固定相上的保留行为 ,优化分离条件。方法聚α 葡糖苷酯、α1 酸性糖蛋白及卵粘蛋白为固定相时 ,流动相分别为正己烷 异丙醇 乙醇 三氟乙酸 (430∶4 5∶2 5∶1)、乙腈 10mmol·L- 1 磷酸盐缓冲液 (NaOH调至pH 7 0 ) (4∶96 )及乙腈 2 0mmol·L- 1 磷酸二氢钾 (三乙胺调至pH 7 0 )(12 7∶87 3)。蛋白柱柱温 2 5℃ ,检测波长均为 2 30nm。结果　本法准确、灵敏、专属性好。Aim To study the chromatographic behavior of cetirizine dihydrochloride on the proteinate- and amylose- based chiral stationary phases so as to optimizate the chromatographic condition of its enantiomers separation. Methods When using amylose-based, α 1-acid glycoprotein and ovomucoid protein chiral stationary phase, the mobile phase was hexane-isopropyl alcohol-alcohol-trifluoroacetic acid (430∶45∶25∶1), acetonitrile-10 mmol·L -1 phosphate buffer solution (adjusted to pH 7.0 with sodium hydroxide) (4∶96) and acetonitrile -20 mmol·L -1 KH 2PO 4 solution (adjusted to pH 7.0 with triethylamine) (12.7∶87.3), respectively. The temperature of proteinate column was 25 ℃ . The detective wavelength was 230 nm. Results The two enantiomers could be separated on the two kinds of chiral stationary phases without derivatization and the resolution was above 2.0. The methods developed on the two kinds of chiral stationary phases are accurate, sensitive and specific. Conclusion Both the proteinate- and amylose-based chiral stationary phases can be used to separate the enantiomers of cetirizine.

关 键 词：蛋白质 纤维素衍生物 手性固定相分离 盐酸西替利嗪对映体 心律失常 

分 类 号：R972.2[医药卫生—药品]