红花RP-HPLC指纹图谱的建立及其质量研究  

Establishment of chromatographic fingerprint and quality assessment of Carthamus tinctorius L.by high performance liquid chromatography

作　　者：赵明波[1] 邓秀兰[2] 王亚玲[1] 卢敏 屠鹏飞[1] 

机构地区：[1]北京大学药学院,北京100083 [2]北京中医药大学基础医学院,北京100029 [3]浙江永宁制药厂,浙江台州318020

出　　处：《药学学报》2004年第3期212-216,共5页Acta Pharmaceutica Sinica

基　　金：国家经贸委中药材生产扶植资金项目(国经贸医药2 0 0 0 5 6号 )

摘　　要：目的　建立红花RP HPLC指纹图谱分析法 ,研究不同产地红花药材的质量。方法　采用梯度洗脱的方法进行色谱分离 ,使用“计算机辅助相似度评价软件”进行数据处理 ,对不同产地红花药材指纹图谱的相似度进行比较分析。结果　不同产地红花药材指纹图谱相似度较好 ,但仍有少数产地的药材指纹图谱中有明显差别。结论　采用RP HPLC方法控制药材的指纹图谱 ,方法重现性好 ,用于红花的质量评价切实可行。不同产地红花药材化学组成相似 ,其相对比例较稳定。Aim To establish chromatographic fingerprint of Carthamus tinctorius L. by RP-HPLC in order to control the quality of Carthamus tinctorius L. Methods The gradient elution mode was applied in chromatographic separation, and data were analysed by “Computer Aided Similarity Evaluation” software to compare the quality of Carthamus tinctorius L. samples from different habitats. Results Samples from different habitats were of high similarity, though a few samples showed evident difference in fingerprint graphics. Conclusion The RP-HPLC fingerprint method is repeatable, feasible in analysis of Carthamus tinctorius L. and can be used in quality assessment of Carthamus tinctoriu s L.. Chemical components in Carthamus tinctorius L. samples from various habitats are similar, and their ratios between each other are stable.

关 键 词：红花 RP-HPLC 指纹图谱 质量 成药生产 临床处方 

分 类 号：R286[医药卫生—中药学] 32[医药卫生—中医学]