密花石斛的HPLC/UV/MS指纹图谱研究  

Studies on fingerprints of Dendrobium densiflorum by HPLC/UV/MS

作　　者：张尊建[1] 王源园[1] 李茜[1] 王兴旺 徐向阳 张蕙 

机构地区：[1]中国药科大学分析测试中心,江苏南京210009 [2]金陵药业股份有限公司技术中心,江苏南京210009

出　　处：《中草药》2004年第4期393-395,共3页Chinese Traditional and Herbal Drugs

摘　　要：目的　采用 HPL C/ U V/ MS法对密花石斛进行指纹图谱研究。方法　采用 Agilent Zorbax SB- C1 8柱 ;甲醇和水为流动相进行梯度洗脱 ;流速为 1.0 m L/ min。结果　得到分离度较好的密花石斛 HPL C/ U V指纹图谱 ,标示了 9个共有峰 ,并用 HPL C/ MS对其部分色谱峰进行了初步定性。结论　该方法可用于密花石斛指纹图谱的测定 ,并为其全面质量控制提供参考。Object To establish a method for studying the fingerprints of Dendrobium densiflorum Lindl. ex Wall. by HPLC/UV/MS. Methods Separation was performed on Agilent Zorbax SB-C 18 column. Gradient elute was performed by the mobile phase consisting of methanol and water with the flow rate of 1.0 mL/min. Results Perfect fingerprints were obtained by HPLC/UV which can be used for the evaluation of D. densiflorum. Nine common peaks in the fingerprints were confirmed, and qualitative analysis of some other peaks were carried on by HPLC/MS. Conclusion The method can be applied to the quality control and studying on the chemical constituents of D. densiflorum crude drug.

关 键 词：密花石斛 指纹图谱 HPLC/UV/MS 

分 类 号：R286.6[医药卫生—中药学] R286.02[医药卫生—中医学]