脑源性神经营养因子在激活态雪旺细胞中表达的初步实验研究  

Primary experimental study of brain-derived neurotrophic factor(BDNF)expression in activated Schwann cell

作　　者：劳杰[1] 姜良福[1] 顾玉东[1] 丁小燕[2] 

机构地区：[1]复旦大学上海市手外科研究所,上海200040 [2]中国科学院上海细胞生物学研究所

出　　处：《中华手外科杂志》2003年第2期109-111,共3页Chinese Journal of Hand Surgery

基　　金：上海市教育基金会"曙光计划资助项目" ;国家自然科学基金资助项目 (30 1 70 965) ;国家 973创伤基础基金资助项目(G1 9990 542 0 2 ) ;国家留学归国人才基金资助项目 ;中国博士后基金资助项目 (2 0 0 2 0 31 1 74)

摘　　要：目的　分析激活态雪旺细胞的脑源性神经营养因子 (brain -derivedneurotrophicfactor ,BDNF)表达及其对神经再生的作用。方法　选用 2 0只SD大鼠 ,将大鼠右侧正中神经及尺神经在腋部切断进行激活 (实验组 ) ,左侧的正常神经为 (对照组 )。采用双酶消化法获取雪旺细胞并进行激活后提取mRNA ,应用RT PCR的方法比较BDNFmRNA的变化。结果　激活态雪旺细胞BDNFmRNA表达比对照组明显增多。结论　激活态雪旺细胞分泌神经营养因子BDNF增多 ,具有促进神经再生的作用。Objective To ananlyse BDNF expression in activated Schwann cell and its effect on nerve regeneration. Methods The right median and ulnar nerves were transected at the axillary level for activation of Schwann cells (experimental group) in 20 SD rats, with their left median and ulnar nerves as control group. Schwann cells were obtained by double kinases digestion and mRNA was distilled from Schwann cells.RT PCR was used to compare the changes of BDNFmRNA. Results The expression of BDNFmRNA in activated Schwann cells is much more than that in the normal Schwann cells. Conclusion Activated Schwann cell improves the serection of BDNF which can promote nerve regeneration.

关 键 词：脑源性神经营养因子 实验 神经再生 激活态雪旺细胞 神经缺损 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]