去卵巢山羊腰椎骨计量学指标的变化  

Bone histomorphometry study on lumbar vertebrae microstructure of ovariectomized goats

作　　者：贺颖[1] 孙晓村[1] 陈槐卿[2] 翁玲玲[2] 郑虎[2] 邱明才[1] 

机构地区：[1]天津医科大学总医院内分泌科,北京军区总医院内分泌科300052 [2]华西医科大学骨质疏松研究中心

出　　处：《中华妇产科杂志》2003年第7期405-408,共4页Chinese Journal of Obstetrics and Gynecology

摘　　要：目的　探讨用山羊制作绝经后骨质疏松动物模型的可行性。方法　切除山羊的双侧卵巢 (OVX) ,制备腰椎不脱钙骨切片 ,应用骨组织形态计量学方法 ,观察正常对照组 (3只 ,无手术处理 )、假手术组 (3只 ,开腹后缝合切口 )、OVX术后 6个月组 (4只 )、术后 18个月组 (5只 )腰椎骨计量学指标的变化。结果　OVX术后 6、18个月组的骨小梁体积占全部骨组织体积的百分比 [(7 9± 1 7)与 (5 8± 0 7) % ]、骨小梁体积占海绵骨体积的百分比 [(12 7± 1 8)与 (9 9± 0 6 ) % ]、平均骨小梁板密度 [(1 0± 0 2 )与 (1 1± 1 1)个 /mm]显著减少 (P <0 0 1) ;平均骨小梁板厚度 [(12 9± 4 5 )与 (95± 15 ) μm]也显著减少 (P <0 0 5 ) ;骨小梁表面和体积之比 [(16 6± 4 6 )与 (2 1 4± 3 1)mm2 /mm3 ]与平均骨小梁板间隙 [(10 0 4± 2 33)与 (95 3± 10 3) μm]则显著增加 (P <0 0 1) ;四环素单标记、双标记、1/ 2单标记与双标记表面之和占全部骨小梁表面的百分比 [(19 5± 3 8)与 (17 5± 1 0 ) %、(16 4± 3 3)与 (13 9± 2 5 ) %、(2 6 2± 5 1)与 (2 2 7± 2 9) % ],平均类骨质宽度 [(11 6± 1 1)与(12 0± 1 0 ) μm]、骨矿化延迟时间 [(2 3 6± 8 2 )与 (2 3 0± 6 1)d]。Objective To demonstrate the histomorphometric changes of postmenopausal osteoporosis (PMO) goat model after bilateral ovariectomy(OVX) Methods Fifteen female goats were randomly enrolled into four groups: 3 goats without any treatment(group C),3 goats sham operated only(group S),4 goats 6 months after OVX(group OVX6M), 5 goats 18 months after OVX (group OVX18M) On the 21st and 9th day prior to sacrifice, tetracycline was given to them in order to label the bone for dynamic study The first lumbar vertebrae from each goat were reserved for bone histomorphometric study Results As compared with group C and S, the trabecular bone volume (TBV)/ total tissue volume,TBV/sponge bone volume, mean trabecular plate thickness(MTPT) and mean trabecular plate density in OVX6M and OVX18M groups were significantly lower ( P <0 01, but MTPT: P <0 05), while the mean trabecular plate space,surface/volume ratio of the trabecular bone, single labelled surfaces, double labelled surfaces , Sfaces (d+1/2s), mean osteoid seam width, mineralization lag time, speed of bone formation in the tissue levela and osteoid maturation period were significantly higher( P <0 01) Conclusion In OVX goats, bone loss accelerated, resulting in destroyed bone microstructure, fasten frequency of activation and high bone turnover Therefore, the bone resorption process exceeds over the bone formation, successfully establishing a high turnover osteoporotic metabolic model

关 键 词：卵巢切除术 骨质疏松 绝经后 骨密度 腰椎 山羊 

分 类 号：R58[医药卫生—内分泌]