剂量与极低频电磁场对细胞因子诱导神经干细胞分化的影响  

DIFFERENTIATION OF NEURAL STEM CELLS INDUCED BY CYTOKINES VARIES WITH DOSE AND ELECTROMAGNETIC FIELD EXPOSURE

作　　者：李怡[1] 邢萱[2] 赵仑[3] 娄淑杰[1] 何成[1] 路长林[1] 

机构地区：[1]第二军医大学神经生物学教研室 [2]第二军医大学数理学教研室,上海200433 [3]第二军医大学航天医学工程研究所,北京100094

出　　处：《解剖学报》2003年第4期344-348,共5页Acta Anatomica Sinica

基　　金：国家自然科学基金资助项目 ( 3 0 170 469)

摘　　要：目的　观察剂量及极低频电磁场对睫状神经营养因子 (CNTF)、白细胞介素 1α(IL 1α)诱导新生大鼠中脑神经干细胞 (NSC)分化的影响。　方法　NSC接受不同剂量CNTF、IL 1α和极低频电磁场处理 5d ,利用神经元特异性标记物微管相关蛋白 2ab(MAP2ab) ,通过免疫荧光化学染色检测神经干细胞神经元向的分化百分比。结果与对照组相比 ,小剂量CNTF和IL 1α组NSC神经元向分化百分比增加 ;大剂量CNTF组该比例明显降低 (P <0 0 1)。施加电磁场后 ,小剂量CNTF的促进效应增强 ,尤其是电磁场可使大剂量CNTF的神经元向分化抑制作用转变为促进作用。电磁场抑制了小剂量IL 1的促进作用 ,不影响大剂量IL 1的诱导结果。　结论　细胞因子对NSC的诱导分化结果与剂量有关。极低频电磁场与细胞因子的共同效应不是简单协同或拮抗 ,说明生物磁场在神经系统发育中的作用不可忽视 ,电磁场可能成为调控NSCs分化的新手段。Objective To examine wether cytokine dose and extremely low\|frequency electromagnetic field(ELF\|EMF) would influence the effects of ciliary neurotrophic factor (CNTF) and interleukin\|1 alpha(IL\|1α) on the differentiation of neural stem cells(NSCs) from midbrains of new\|born rats. Methods Differentiation of NSCs,exposed or unexposed to ELF\|EMF,was induced by CNTF or IL\|1α at different concentration for 5 days.Cells then were fixed and processed for immunofluorescent staining to detect the neuron\|specific marker,microtubule\|associated protein 2ab(MAP2ab),thus the percentage of MAP2ab\++ cells was calculated. Results In contrast with the control,low\|dosed CNTF and IL\|1α increased the percentage of neuron\|fated cells while high\|dosed CNTF significantly decreased the percentage of MAP2ab\|positive cells( P <0\^01).ELF\|EMF exposure enhanced the stimulating effect of low\|dosed CNTF;The most exciting finding was that the EMF converted the inhibitory effect of high\|dosed CNTF on neurogenesis to the stimulative effect.The EMF diminished the promoting effects of low\|dosed IL\|1α as well as failed to influence the high\|dosed IL\|1α effect.Conclusion\ Inductive effects of cytokines on NSCs differentiation are dose\|related.Coeffects of ELF\|EMF and cytokines would not be simply added up or subtracted,implying that the role of organism EMF during CNS development should not be ignored.EMF may be a promising strategy for manipulating NSCs differentiation.\;[

关 键 词：剂量 极低频电磁场 细胞因子 诱导 神经干细胞 细胞分化 

分 类 号：R329.21[医药卫生—人体解剖和组织胚胎学]