原代培养大鼠嗅球成鞘细胞Nogo(N-18)的共聚焦激光扫描显微镜观察  

EXPRESSION OF THE Nogo PROTEIN IN THE PRIMARY CULTURED ENSHEATHING CELLS FROM OLFACTORY BULB OF ADULT RAT BY CONFOCAL LASER SCANNING MICROSCOPE

作　　者：王君[1] 王珂[1] 周长满[1] 于恩华[1] 

机构地区：[1]北京大学医学部解剖学系,北京100083

出　　处：《解剖学报》2003年第4期363-366,共4页Acta Anatomica Sinica

基　　金：北京大学 ( 985 )行动计划项目 ( 70 7 68914 3 ) ;北京市自然科学基金 ( 70 2 2 0 18)资助

摘　　要：目的　观察原代培养的成年大鼠嗅球成鞘细胞 (OECs)Nogo(N 18)蛋白的表达 ,探讨Nogo与成鞘细胞促进神经再生作用的关系。　方法　原代培养嗅球OECs,采用免疫组织化学和双标免疫荧光细胞化学技术 ,结合激光共聚焦扫描显微镜观察。　结果　原代培养的嗅球OECs的Nogo(N 18)免疫细胞化学反应呈阳性 ,Nogo(N 18)蛋白主要分布于胞浆 ,而在胞膜及突起分布较少。　结论　嗅球 (OECs)含有Nogo A蛋白 ,提示NogoObjective Studying the expression of the Nogo(N\|18) in olfactory ensheathing cells(OECs) to investigate the functional relationship between Nogo and the OECs in promoting the regeneration of neurite axon. Methods The expression of the Nogo(N\|18) in the primary cultured ensheathing cells from olfactory bulb of the adult rats was studied with the method of immunocytochemical staining and double\|immunofluorescence staining examed under the confocal laser scanning microscope. Results The Nogo\|A protein was located in primary cultured ensheathing cells from the olfactory bulb.The protein was mainly distributed in the cytoplasm.The member and processes were less stained.Conclusion\ The ensheathing cells in vitro contain Nogo protein.It suggests that Nogo protein is not a depressive factor in the axon regeneration of olfactory system.\;[

关 键 词：嗅神经成鞘细胞 N0g0蛋白 细胞培养 共聚焦激光扫描 大鼠 

分 类 号：R322.8[医药卫生—人体解剖和组织胚胎学]