维甲酸诱导大鼠胚胎脑神经干细胞P450RAI基因的表达  

EXPRESSION OF P450RAI IS INDUCED BY RETINOIC ACID IN NEURAL STEM CELLS FROM EMBRYONIC BRAINS OF RATS

作　　者：崔景彬[1] 王俊萍[1] 李开荣[1] 鄢文海[2] 王琳娜[1] 

机构地区：[1]郑州大学基础医学院生物化学教研室,郑州450052 [2]郑州大学病理生理学教研室,郑州450052

出　　处：《解剖学报》2003年第4期375-378,共4页Acta Anatomica Sinica

基　　金：河南省医学科技创新人才工程资助项目( 2 0 0 2 10 7)

摘　　要：目的　探索全反式维甲酸 (RA)对神经干细胞和分化细胞P4 5 0RAI表达的影响。　方法　从大鼠胚胎脑中分离神经干细胞 ,用EGF促进其增殖 ,再用不同浓度的RA单独处理 ,在不同时间用RT PCR测定神经干细胞及分化细胞的P4 5 0RAI表达水平。　结果　 1μmol LRA处理神经干细胞 ,P4 5 0RAImRNA水平最高。 1μmol L时2h就可检测到神经干细胞P4 5 0RAImRNA的生成 ,4～ 12h达到高峰 ,随后迅速下降 ,2 4h几乎检测不到P4 5 0RAImRNA。间断给予RA ,P4 5 0RAI的表达随之下降。在EGF诱导生成的分化细胞中无P4 5 0RAI表达 ,而在RA处理的分化细胞中有少量表达。　结论　神经干细胞表达P4 5 0RAI对RA有浓度依赖性并需要RA的持续存在 ,随着神经干细胞分化为神经细胞 。Objective To study the effect of expression of P450RAI induced by all\|trans retinoic acid(RA) on neural stem cells(NSCs) and differentiated cells in vitro. Methods NSCs were isolated from rat embryonic brains,cultured and induced to proliferate with epidermal growth factor(EGF). Then,NSCs were treated with various concentrations of RA.At various time points,RT\|PCR was used to determine the levels of P450RAI mRNA in NSCs and differentiated cells. Results The levels of P450RAI mRNA in NSCs was the highest following 1?μmol/L treatment with different concentrations of RA.At 2?h following 1?μmol/L RA treatment,low levels of P450RAI mRNA were detectable,and the highest between 4 and 12?h of treatment,bu rapidly declined afterwards and were barely detectable by 24?h.RA was administrated with interruption in culture,expression of P450RAI was also declined.There was no expression of P450RAI in the differentiated cells with EGF but low levels with RA.Conclusion\ These findings suggest that expression of P450RAI in NSCs is dependent on both the concentrations of RA and the continuous presence of RA.The levels of P450RAI mRNA decline when NSCs are induced to differentiate into neural cells.\;[

关 键 词：维甲酸 P450RAI RT-PCR 神经干细胞 基因表达 胚胎 大鼠 

分 类 号：R322.85[医药卫生—人体解剖和组织胚胎学] R338.2[医药卫生—基础医学]