Thapsigargin对SLE患者T细胞TCR/CD3复合物介导的[Ca^(2+)]i反应的影响  

Effect of thapsigargin on TCR/CD3 complex-mediated [Ca^(2+)]i responses in T cells of SLE

作　　者：杨庆永[1] 廖元兴[1] 王宗发[2] 杨慧兰[1] 

机构地区：[1]广州军区广州总医院皮肤科,510010 [2]第一军医大学南方医院皮肤科,广州510515

出　　处：《中国麻风皮肤病杂志》2003年第5期421-423,共3页China Journal of Leprosy and Skin Diseases

摘　　要：目的 :　证实SLE患者T细胞功能异常是否与TCR CD3复合物介导的 [Ca2 +]i反应异常有关 ,以及 [Ca2 +]i反应异常的原因。方法 :　用CD3单抗与羊抗鼠二抗IgG相关联刺激T细胞 ,并用Thapsigargin干预后 ,分别用粘附细胞仪连续观察 10minT细胞 [Ca2 +]i的变化 ,并评价 [Ca2 +]i反应与CD3分子表达的相关性。结果 :　正常人和SLE患者T细胞 [Ca2 +]i反应的基准值相似 (P =0 .10 5 ) ;SLE患者T细胞的[Ca2 +]i反应高峰值、平台值明显高于正常对照 (P <0 .0 0 1,P <0 .0 0 1) ;加入Thapsigargin后二者 [Ca2 +]i反应无显著差异 ,二者的T细胞CD3阳性率无差异 (P =0 .6 6 5 )。结论 :　SLE患者T细胞TCR CD3介导的[Ca2 +]i反应存在异常 ,并且这种异常不是因为胞内Ca2 +库排空后跨膜Ca2 +内流不同所致。Objective: To determine whether the primary dysfunction of T cells of SLE was related to abnormal [Ca 2+]i responses, and the reason of the abnormal [Ca 2+]i response. Methods: After cross-linking of anti-CD3 mAb to sheep anti-mouse IgG and stimulation of T cells, the changes of free calcium ion within T cells and the changes under interference of thapsigargin were observed successively for 10 minutes by an adhesion cytometry. The relation between [Ca 2+]i response in T cells of SLE and CD3 expression was evaluated.Results:The baseline [Ca 2+]i response in T cells of SLE patients was similar to that in normal control (P=0.105). The peak and plateau [Ca 2+]i responses were significantly higher in the group of SLE patients than in normal control (P<0.001,P<0.001). [Ca 2+]i responses were similar in both individuals after adding thapsigargin, and the percentage of CD3 + cells was similar in both groups (P=0.665). Conclusion: TCR/CD3-mediated [Ca 2+]i response in T cells of SLE was abnormal, which can not be attributed to the difference in Ca 2+ influx across the cell surface membrane after emptying intracellular Ca 2+ stores.

关 键 词：THAPSIGARGIN SLE T细胞 TCR/CD3复合物 钙离子 信号转导 系统性红斑狼疮 

分 类 号：R593.241[医药卫生—内科学]