RANTES在银屑病患者角质形成细胞中的表达及其作用  

The role of RANTES expressed by keratinocytes from psoriatic lesions

作　　者：唐玲[1] 李泉[1] 陈洁[1] 于益芝[2] 唐跃琼[1] 郑茂荣[1] 

机构地区：[1]第二军医大学附属长海医院皮肤科,上海200433 [2]第二军医大学免疫学研究所

出　　处：《中国麻风皮肤病杂志》2003年第5期426-427,共2页China Journal of Leprosy and Skin Diseases

基　　金：国家自然科学基金 (30 2 71 2 0 2 ) ;国家 863计划(2 0 0 1AA2 1 50 91 )

摘　　要：目的 :　探讨银屑病皮损局部T淋巴细胞高度浸润的原因 ,并分析了趋化因子RANTES在此过程中的作用。方法 :　培养银屑病患者皮损处角质形成细胞 ,通过微孔小室实验检测其上清液对T淋巴细胞的趋化功能 ;通过酶联免疫吸附 (ELISA)法检测上清液中RANTES的表达。结果 :　银屑病皮损处角质形成细胞培养上清液对T淋巴细胞的趋化能力明显强于正常对照组 ;其分泌的RANTES水平也高于正常人。结论 :　银屑病患者皮损局部T淋巴细胞的大量浸润 ,部分是由于角质形成细胞具有较强的趋化T淋巴细胞能力 ,而银屑病角质形成细胞中高表达的RANTES可能是发挥此作用趋化因子之一。Objective: To investigate the mechanisms of focal accumulation of T lymphocytes in psoriatic lesions and analyze the role of chemotaxis RANTES in the process.Methods: Chemotaxis of psoriatic lesional keratinocytes was detected by micropore loculus test. The concentration of RANTES in cultured supernatants of psoriatic lesional keratinocytes was determined by ELISA. Results: The chemotaxis of psoriatic lesional keratinocytes to T lymphocytes was stronger than that of controls. The concentration of RANTES in the cultured supernatants from psoriatic lesional keratinocytes also increased. Conclusion: Focal accumulation of T lymphocytes in psoriatic lesions may be partly related to stronger chemotaxis of psoriatic lesional keratinocytes to T lymphocytes. RANTES secreted by psoriatic keratinocytes may play one of key roles in the course.

关 键 词：RANTES 银屑病 角质形成细胞 T淋巴细胞 发病机制 

分 类 号：R758.63[医药卫生—皮肤病学与性病学]