骨组织工程研究中的美学考量  

A aesthetic study of bone tissue engineering

作　　者：雷德林[1] 羊书勇[1] 吴凡[1] 陈书军[1] 刘彦普[1] 杨维东[1] 毛天球[1] 

机构地区：[1]第四军医大学口腔医学院颌面外科,陕西省西安市710032

出　　处：《中国美容医学》2003年第3期243-244,共2页Chinese Journal of Aesthetic Medicine

摘　　要：目的:分别以珊瑚羟基磷灰石(CHA)、藻酸钙为成骨细胞载体,观察裸鼠体内的构建特定形态和可注射组织工程骨的可行性。方法:取兔髂骨,获取骨髓基质干细胞,经体外扩增培养诱导后,获得骨髓基质成骨细胞,分别与CHA和藻酸盐复合,植入和注射于裸鼠背部皮下,6、8周后进行大体观察、组织学和X线检查,观察软骨和骨的形成。以单纯CHA和藻酸盐植入作为对照。结果:植入CHA/成骨细胞组和注射藻酸盐/成骨细胞复合物组均有骨样组织形成,具备骨髓腔样结构,而对照组无骨形成。结论:CHA和藻酸盐均是较好的骨组织工程支架材料,复合成骨细胞后可构建特定形态和可注射组织工程骨。Objective: We injected the osteoblasts/alginate composites and implanted osteoblasts/CHA composites into the dorsal subcutaneous tissue of nude mice and observed the formation of bone. Methods: Rabbit bone marrow stem cells were cultured and expanded in vitro and osteoblasts were harvested. Some of the osteoblasts were mixed with algi-nate others were combined with special shaped CHA. The former were injected and the later were implanted into the dorsal subcutaneous tissue of nude mice. 4 and 8 weeks after operation, the animals were sacrificed and new bone regeneration was investigated by X-ray examination, histological observation and SEM. Results: In the two experimental teams, new bone tissue was formed and no bone formation was observed in the control team. Conclusion: Both alginate calcium and CHA can be used as scaffolds of tissue engineered bone.

关 键 词：骨 组织工程 美学 骨髓基质成骨细胞 珊瑚羟基磷灰石 藻酸盐 可注射性 

分 类 号：R322.71[医药卫生—人体解剖和组织胚胎学]