转化生长因子-β诱导活化蛋白-1活化及促进NIH3T3细胞α2Ⅰ型胶原表达的研究  

TGF-β induces AP-1 DNA binding activity and promotes the expression of collagen mRNA in NIH3T3 cell

作　　者：郝天智[1] 梁华平[1] 吕凤林[1] 徐祥[1] 国华[1] 王付龙 

机构地区：[1]第三军医大学附属大坪医院野战外科研究所

出　　处：《中华实验外科杂志》2003年第5期406-407,共2页Chinese Journal of Experimental Surgery

基　　金：国家重点基础研究规划项目 (G1 9990 542 0 3) ;国家自然科学基金资助项目 (C0 30 2 0 4 ) ;重庆市卫生局课题 (2 0 0 1 2 0 0 3)

摘　　要：目的　研究活化蛋白 1(AP 1)在转化生长因子 β(TGF β)促进Ⅰ型胶原基因表达中的作用 ,探讨病理性瘢痕的形成机制。方法　用 5 μg/LTGF β刺激鼠成纤维细胞株NIH 3T3细胞 ,分别采用凝胶迁移变动分析 (EMSA)和逆转录 聚合酶链反应 (RT PCR)技术研究TGF β对AP 1的活化及其对α2 Ⅰ型胶原mRNA水平的影响。结果　 5 μg/LTGF β刺激NIH3T3细胞 ,0 .5h即可检测到AP 1被活化 ,随时间推移AP 1的活性逐渐增强 ,6h达到峰值 ,而后活性逐渐减弱 ,2 4h仍然可检测到AP 1的活性。用TGF β刺激NIH3T3细胞 ,2 4h后提取总RNA ,经RT PCR分析 ,与对照组相比α2 Ⅰ型胶原mRNA水平明显增高。结论　TGF β刺激NIH3T3细胞 ,可激活AP 1并可以上调α2 Ⅰ型胶原的表达。Objective To investigate the effect of AP 1 on TGF β promoting the expression of typeα2Ⅰcollagen mRNA.Methods NIH3T3 cells were treated with TGF β,and nuclear proteins were extracted,then AP 1 DNA binding activity were analyzed by EMSA; Total RNA was isolated and analyzed by RT PCR.Results AP 1 DNA binding activity could be observed after the cells were treated with TGF β for 0.5 h,and the pick point was at 6 h,and then AP 1 activity decreased greadually,but AP 1 activity could be detected at 24th h; After the cells were treated with TGF β for 24 h,the expression of α2Ⅰ collagen mRNA was increased significantly.Conclusion Not only could TGF β induce AP 1 DNA binding activity,but also it could promote the expression of type α2Ⅰcollagen mRNA relative to untreated control.

关 键 词：转化生长因子-Β 诱导 活化蛋白-1 NIH3T3细胞 α2Ⅰ型胶原 表达 病理性瘢痕 

分 类 号：R602[医药卫生—外科学]