ECL法检测前列腺特异抗原的临床价值  

Clinical Usefulness of Detenmination of Serum PSA Levels with ECL

作　　者：张俊[1] 盛强[1] 丁忠琪 李永培[1] 骆佩芳[1] 霍艳秋[1] 

机构地区：[1]泰州市人民医院核医学科,225300

出　　处：《放射免疫学杂志》2004年第2期152-154,共3页Journal of Radioimmanology

摘　　要：目的 :探讨前列腺特异性抗原 (PSA)浓度测定在前列腺疾病诊断中的应用价值。方法 :采用电化学发光免疫分析 (ECL)法检测 81例健康对照者、5 8例前列腺炎患者、1 2 1例良性前列腺增生 (BPH)患者和 6 9例前列腺癌 (PCa)患者血清中的PSA浓度 ,对检测结果进行统计分析。组间两两比较用t检验 ;良、恶性鉴别诊断界值的确定用ROC曲线。结果 :(1 )前列腺癌组患者血清PSA浓度明显高于其它各组 (p皆 <0 0 0 1 ) ;前列腺增生组部分病人血清PSA浓度升高 (与健康对照组和炎症组比较 p <0 0 0 1 ) ,但局限在一定范围 ,一般不超过 1 0ng/ml;前列腺炎组和健康对照组均在正常范围 (<4ng/ml)。 (2 )ROC曲线下面积为 0 98,当鉴别界值为4ng/ml时 ,灵敏度为 1 0 0 % ,特异度为 77 3% ;界值为 1 8ng/ml时 ,特异度 1 0 0 % ,灵敏度 6 8 1 % ;界值为 7 6ng/ml时 ,灵敏度为 95 7% ,特异度为 90 %。结论 :PSA是筛查前列腺癌的灵敏的肿瘤标志物 ,对良、恶性前列腺疾病有一定的鉴别诊断意义。Objective To investigate the clinical usefulness of serum PSA levels determination with ECL immunoassay for diagnosis of prostatic diseases. Methods Serum PSA levels were determined with electro-chemiluminescence immunoassay (ECLIA) in 81 controls, 58 patients with chronic prostatitis, 121 patients with benign prostatic hypertrophy (BPH) and 69 patients with prostatic carcinoma (PCa). Cut-off value for diagnosis of malignancy was determined with ROC carre. Results (1) Serum PSA levels in patients with PCa were significantly higher than those in all othen subjects tested ( p <0 001). In patients with BPH, serum PSA levels were significantly higher than those in patients with prostatitis and controls ( p <0 001) but usually lower than 10ng/ml PSA levels in patients with prostntitis and controls were within normal range (<4ng/ml). (2) The area under ROC curve was 0.98. Sensitivity and specificity were both high (95.7% and 90%) with 7.6ng/ml set as cut-off value. Conclusion Measurement of serum PSA levels with ECLIA is convenient and accurate for diagnosis of prostatic malignancies.

关 键 词：ECL法 前列腺特异抗原 PSA 前列腺增生 BPH 

分 类 号：R697.3[医药卫生—泌尿科学]