三羟异黄酮对豚鼠心室肌细胞内游离钙浓度的影响(英文)  

Effects of genistein on intracellular free-calcium concentration in guinea pig ventricular myocytes

作　　者：吉恩生[1] 王川[2] 何瑞荣[1] 

机构地区：[1]河北医科大学基础医学研究所生理研究室,石家庄050017 [2]河北医科大学基础医学研究所,药理研究室,石家庄050017

出　　处：《生理学报》2004年第2期204-209,共6页Acta Physiologica Sinica

摘　　要：用激光共聚焦显微镜观察研究三羟异黄酮(genistein,GST)对豚鼠心室肌细胞内游离钙浓度([Ca2+]i)的影响。结果用相对荧光强度(FI-F0/FI0,％)表示。实验结果显示,在正常台氏液、无钙台氏液和正常台氏液中加入3 mmol/l EGTA后,GST(10-40μmol/L)浓度依赖性地降低细胞内钙浓度。蛋白酪氨酸磷酸酶抑制剂正钒酸钠(sodium orthovanadate)和L-型Ca2+通道激动剂Bay K8644可部分抑制正常台氏液时GST的效应。当细胞外液钙浓度由1 mmol/L增加到10 mmol/L而诱发心室肌细胞钙超载时,部分心室肌细胞产生可传播的钙波,GST(40μmol/L)可降低钙波的传播速度和持续时间,最终阻断钙波。以上结果提示,GST降低心室肌细胞内游离钙浓度,此作用与其抑制电压依赖性Ca2+通道、减弱酪氨酸激酶抑制和豚鼠心室肌细胞肌浆网内钙释放有关。The effects of genistein (GST) on intracellular calcium concentration ([Ca2+]i) were investigated in guinea pig ventricular myocytes. [Ca2+]i was detected by confocal microscopy and represented by relative fluorescent intensity (FI-F0) /FI0, %). The results showed that GST (10-40 umol/L) reduced [Ca2+]i in normal Tyrode's solution, Ca2+-free Tyrode's solution and normal Tyrode's solution containing 3 mmol/L EGTA in a concentration-dependent manner. The effects of GST on [Ca2+]i in normal Tyrode's solution were partially inhibited by pretreatment with sodium orthovanadate, a potent inhibitor of protein tyrosine phosphatase, or L-type Ca2+ channel agonist Bay K8644. GST also markedly inhibited the ryanodine-induced [Ca2+]i responses in Ca2*-free Tyrode's solution. When Ca2+ waves were produced by increasing extracellular Ca2+ concentration from 1 to 10 mmol/L, GST (40 umol/L) could block the propagating waves of elevated [Ca2+]i' and reduce the velocity and duration of propagating waves. These results suggest that GST may reduce the [Ca2+]i in isolated guinea pig ventricular myocytes. The inhibition of voltage-dependent Ca2+ channel, tyrosine kinase inhibition, and alleviation of Ca2+ release from SR are possibly involved in the GST effect.

关 键 词：三羟异黄酮 荧光密度 心室肌细胞 细胞内钙 钙通道 钙释放 共聚焦显微镜 

分 类 号：Q952[生物学—动物学]