人新的精子结合蛋白HBRP新功能的研究  

Study on New Function of Human Novel Sperm Binding Protein

作　　者：张杰[1] 刘莹[1] 宗志宏[1] 惠答美[1] 刘奕[1] 于秉治[1] 

机构地区：[1]中国医科大学基础医学院生化教研室,沈阳110001

出　　处：《生殖与避孕》2004年第2期74-77,T003,共5页Reproduction and Contraception

基　　金：国家自然科学基金资助项目(30170987)

摘　　要：目的:探讨新发现的人睾丸组织中与精浆蛋白BSP相关的新基因的生物学功能。方法:利用真核重组表达质粒pcDNA3/HBRP转染人胚肾HEK293细胞,建立稳定的真核表达细胞系,并用放射自显影检测该细胞系的PKC活性。结果:HBRP蛋白对PKC活性有明显的抑制作用。结论:确定了人新的精子结合蛋白HBRP的功能之一是抑制PKC活性。Objective: To study the biological function of novel gene related to bovine seminal plasma protein in human testis. Methods: Transfect HEK293 cell by recombination eukaryotic expression vector pcDNA3/ HBRP,construct stable expression cell line, and detect the activity of protein kinase C (PKC) by radioautogram. Results: The HBRP protein inhibits the activity of PKC obviously. Conclusion.One new function of novel sperm binding protein in human is to inhibit the activity of PKC.

关 键 词：HBRP 真核表达 PKC活性 

分 类 号：R341[医药卫生—基础医学]