亚甲基四氢叶酸还原酶基因C677T多态与2型糖尿病脑梗死的关系  

Relationship between the C677T polymorphism in the methylenetetrahydrofolate reductase gene and cerebral infarction complicated type 2 diabetes

作　　者：周君[1] 李晓霞[2] 张巾超[3] 

机构地区：[1]牡丹江医学院红旗医院神经内科 [2]牡丹江医学院预防医学教研室,黑龙江牡丹江157011 [3]哈尔滨医科大学附属第二医院内分泌科,黑龙江哈尔滨150086

出　　处：《中风与神经疾病杂志》2004年第2期136-138,共3页Journal of Apoplexy and Nervous Diseases

基　　金：黑龙江省自然科学基金 (D-0 3 5 7)

摘　　要：目的　探讨亚甲基四氢叶酸还原酶 (methylenetetrahydrofolate reductase,MTHFR)基因 C6 77T多态与中国东北汉族 2型糖尿病患者合并脑梗死的关系。方法　应用聚合酶链反应 -限制性片段长度多态性 (PCR-RFL P)方法检测了对照组、2型糖尿病无大血管病变组和 2型糖尿病脑梗死组共 2 0 8名的 MTHFR基因 C6 77T多态性分布。结果　经 χ2检验 ,MTHFR基因各基因型频率在对照组、2型糖尿病组和 2型糖尿病脑梗死组之间均无显著性差异 (P>0 .0 5 ) ;各等位基因频率在 3组人群中均无显著性差异 (P>0 .0 5 )。结论　未发现 MTHFR基因C6 77T多态与 2型糖尿病脑梗死之间存在相关关系。Objective To study the relationship between the C677T polymorphism of methylenetetrahydrofolate reductase(MTHFR) gene and cerebral infarction complicated type 2 diabetes of Han nationality in Northeast China. Methods MTHFR gene C677T porlymorphism was detected in 208 subjects,including 69 in control (CON) group,67 in type 2 diabetes mellitus without macrovascular disease (DM2) group and 72 in type 2 diabetes mellitus with cerebral infarction (DM2CI) group by PCR-RFLP technique.Results No significant difference in genotype frequencies and allele frequencies of MTHFR gene was found among the CON group,DM2 group and DM2CI group,by χ 2 test analysis (P>0.05, respectively).Conclusions No association between MTHFR gene C677T porlymorphism and DM2CI was noted in present study.

关 键 词：亚甲基四氢叶酸还原酶基因 C677T多态 糖尿病脑梗死 聚合酶链反应 限制性片段 长度 多态性 

分 类 号：R587.1[医药卫生—内分泌] R743[医药卫生—内科学]