荧光定量PCR在丙型肝炎病毒感染检测中的应用  

Clinicial significance of detection of HCV infection with FQ-PCR

作　　者：余辉[1] 俞丽琴[2] 陈春辉[2] 

机构地区：[1]九江大学医学院微生物学教研室,江西九江332000 [2]九江市第三医院检验科,江西九江332000

出　　处：《现代医学》2004年第2期93-95,共3页Modern Medical Journal

摘　　要：目的　观察荧光定量聚合酶链反应 (FQ -PCR)在丙型肝炎病毒 (hepatitisCvirus ,HCV )感染检测中的临床应用情况。方法　用FQ PCR检测 3 15份怀疑HCV感染的临床血清标本 ,并同时采用酶联免疫吸附测定法 (ELISA)检测抗HCV ,用生化仪测定血清丙氨酸氨基转移酶 (ALT)水平 ,了解样本中HCV RNA含量与抗HCV及ALT的相关性。结果　 3 15份标本中有 2 2 5份HCV RNA含量高于 80拷贝·ml-1,2 5 0份抗HCV阳性 ,14 5例ALT异常 ,HCV RNA含量与抗HCV阳性率间有显著的相关性 (r =0 .682 ,P =0 .0 0 2 ) ;HCV RNA含量与ALT异常率间也有显著的相关性 (r =0 .92 3 ,P =0 .0 3 2 )。结论　FQ PCR技术检测HCV RNA特异性强 ,灵敏度高 ,具有良好的临床应用价值。Objective To investigate the clinical application of the fluorescence quantitative PCR (FQ?PCR) in the detection of hepatitis C virus( HCV) infection.Methods Three hundred and fifteen sera from patients clinically diagnosed hepatitis C were detected by FQ?PCR,antibody to HCV were detected with ELISA,and alanine transferase(ALT)were detected by biolchemical instruments.Spearman correlation analysis was used to confirm the correlation between the HCV?RNA copies and the anti?HCV titers and ALT standard.Results HCV?RNA copies in 225 of 315 samples were more than 80 copies·ml -1 ,while 250 of 315 samples were detected anti?HCV positive,and 145 of 315 were detected abnomal at ALT.There were good correlation both between HCV?RNA copies and anti?HCV positive rate ( r =0.682, P= 0.002),and between HCV?RNA copies and ALT abnormal rate ( r =0.923, P =0.032).Conclusion FQ?PCR is a specific and sensitive method for the detection of HCV infection.It can be widely used in clinical detection.

关 键 词：荧光定量PCR 丙型肝炎病毒感染 检测 FQ-PCR (ELISA 

分 类 号：R512.62[医药卫生—内科学] R446.112[医药卫生—临床医学]